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2 protocols using quercetin 3 d glucoside

1

Analytical Characterization of Natural Compounds

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CMC was purchased from CP Kelco (Äänekoski, Finland). Sucrose was a product of Gram-mol (Zagreb, Croatia). Trehalose was obtained from Hayashibara doo (Nagase group, Tokyo, Japan). Hydrochloric acid was from Carlo Erba Reagents (Sabadell, Spain). Sodium carbonate was from T.T.T. (Sveta Nedelja, Croatia). Folin–Ciocalteu reagent and potassium persulfate were from Kemika (Zagreb, Croatia). Trolox, 2,2-diphenyl-1-picrylhydrazil, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and most of the standards used (rutin, quercetin, quercetin-3-D-glucoside, chlorogenic acid) were products of Sigma-Aldrich (St. Louis, MO, USA), while the standards of cyanidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-arabinoside, neochlorogenic acid, and quercetin-3-D-galactoside were products of Extrasynthese (Genay, France). Cupric chloride, neocuproine, and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were purchased from Acros Organic (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade, >85%) was from Fisher Scientific (Longhborough, UK).
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2

Evaluation of Analgesic Compounds

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The following drugs and reagents were used: Indomethacin -Ind (Sigma-Aldrich Inc., St. Louis, MO, USA), morphine sulfate -Mor (Cristália, SP, Brazil), acetic acid and formalin (Merck KGaA, Darmstadt, Germany) . Dimethylsulfoxide (DMSO): physiological saline, 1:50, was used in all experiments as the control. Flavonoids and cinnamic derivatives were used as standards being chlorogenic acid, quercetin-3-Dgalactoside, quercetin-3-D-glucoside e isorhamnetin-3-O-galactoside (Sigma Aldrich®). All the solvents for Ultra-fast liquid chromatography (UFLC) were of HPLC grade. All other reagents were of analytical grade.
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