Log In Sign up
The largest database of trusted experimental protocols

Cd19 car detection reagent biotin

Manufactured by Miltenyi Biotec
Visit Supplier

The CD19 CAR Detection Reagent (Biotin) is a laboratory tool used to detect the presence of CD19-specific chimeric antigen receptors (CAR) on the surface of cells. It is a biotin-labeled antibody that binds to the CD19 CAR, allowing for its identification and quantification through further analytical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Novoexpress software, by Agilent Technologies (2 mentions) Fitc labeled human cd19, by ACROBiosystems (2 mentions) Streptavidin apc cy7, by Abcam (2 mentions) Dylight 650, by Thermo Fisher Scientific (2 mentions) Novocyte 2060, by Agilent Technologies (2 mentions) Fluorescein isothiocyanate (fitc), by BioLegend (1 mentions) Tigit pe cy7, by BioLegend (1 mentions) Cd62l apc, by BioLegend (1 mentions) Cd3 apc cy7, by BioLegend (1 mentions) Cd19 pe, by BioLegend (1 mentions) Cd274 pd l1 apc, by BioLegend (1 mentions) Cd45ra pe, by BioLegend (1 mentions) Sh800, by Sony (1 mentions) Fluorescein isothiocyanate (fitc), by Abcam (1 mentions) Anti human cd107a pe, by BD (1 mentions) Anti human cd8 pe cy7, by BD (1 mentions) Sh800 cell sorter, by Sony (1 mentions) Pe anti human cd19, by BioLegend (1 mentions) Apc anti human cd33, by BioLegend (1 mentions)

Spelling variants of this product

CD19 CAR Detection Reagent (13 citations) CD19 CAR - Biotin (2 citations)

2 protocols using cd19 car detection reagent biotin

1

Characterization of CAR T-cells and PD1-VHH

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies against the following markers were used in this study (all anti-human): CD3 APC/Cy7 (Biolegend), CD19 PE (Biolegend), PD-1 (CD279) PE (BD Biosciences) and FITC (Biolegend), PD-L1 (CD274) APC (Biolegend), CD45RA PE (Biolegend), CD62L APC (Biolegend), TIGIT PE/Cy7 (Biolegend). The CAR molecules were detected by staining with CD19 CAR Detection Reagent (Biotin) (Miltenyi Biotec), or FITC-Labeled Human CD19 (Acro Biosystems), for primary staining and streptavidin-APC/Cy7 or FITC (Abcam) for visualization. The PD1-VHH-Fc-PDGFR molecules were detected using goat cross-absorbed anti-human IgG antibody conjugated with DyLight650 (Thermo Fisher Scientific). Flow cytometric analysis was performed using NovoCyte 2060 (ACEA Biosciences) instrument, data were analyzed with FlowJo X10 (FlowJo) and NovoExpress Software (ACEA Biosciences). Jurkat-Lucia™ NFAT and Nalm-6 were transduced with the pLV2 PD-1 and pLV2 PD-L1 lentiviruses, respectively, and positive cells were sorted using SH800 (Sony) instrument.
Kalinin R.S., Ukrainskaya V.M., Chumakov S.P., Moysenovich A.M., Tereshchuk V.M., Volkov D.V., Pershin D.S., Maksimov E.G., Zhang H., Maschan M.A., Rubtsov Y.P, & Stepanov A.V. (2021). Engineered Removal of PD-1 From the Surface of CD19 CAR-T Cells Results in Increased Activation and Diminished Survival. Frontiers in Molecular Biosciences, 8, 745286.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorophore-conjugated mAbs were used: antihuman CD33 APC (Biolegend), anti-human CD123 PE (Biolegend), anti-human CD19 PE (Biolegend), anti-human IL13Ra2 FITC (R&D Systems), anti-human HER2 APC (R&D Systems), anti-human CD8-PE-Cy7 (BD Biosciences), anti-human CD4-APC-Cy7, anti-human CD107a-PE (BD Biosciences). The CAR molecules were detected by staining with CD19 CAR Detection Reagent (Biotin) (Miltenyi Biotec), FITC-Labeled Human CD19 (Acro Biosystems), goat anti-human IgG antibody conjugated with DyLight650 (Thermo Fisher Scientific) or recombinant protein L conjugated with FITC (Acro Biosystems), and streptavidin-APC/Cy7 (Abcam). The AVs surface proteins were detected as follows: the AVs were incubated with monoclonal antibodies or isotype controls for 30 min at 4 °C. Subsequently, the AVs were washed once with 500 µL PBS and analyzed by flow cytometry. NovoCyte 2060 (ACEA Biosciences) instrument was used for flow cytometry, data were analyzed with FlowJo X10 (FlowJo) and NovoExpress Software (ACEA Biosciences). Cell lines were sorted by SH800 Cell Sorter (Sony).
Ukrainskaya V., Rubtsov Y., Pershin D., Podoplelova N., Terekhov S., Yaroshevich I., Sokolova A., Bagrov D., Kulakovskaya E., Shipunova V., Deyev S., Ziganshin R., Chernov A., Telegin G., Maksimov E., Markov O., Oshchepkova A., Zenkova M., Xie J., Zhang H., Gabibov A., Maschan M., Stepanov A, & Lerner R. (2021). Antigen-Specific Stimulation and Expansion of CAR-T Cells Using Membrane Vesicles as Target Cell Surrogates. Small (Weinheim an der Bergstrasse, Germany), 17(45).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!