Antibodies used in this analysis were: Hic-5 611164, β1 integrin clone 9EG7 553715, CD29 610467, ILK 611803 (BD Biosciences, Franklin Lakes, NJ USA); tensin1 NBP1-84129 (Novus Biologicals, Littleton, CO, USA); phosphotyrosine clone 4G10 05-321 (Millipore); talin T3287, fibronectin F3648, α-tubulin clone DM1A T9026, α-actinin A5044, tensin1 SAB200283 (Sigma-Aldrich, St. Louis, MO, USA); phosphotyrosine clone P-Tyr-100 9411 (Cell Signaling, Danvers, MA, USA); β1 integrin clone 12G10 ab30394 (Abcam, Cambridge, MA, USA); LAMP1 bs-1970R (Bioss, Woburn, MA, USA); Epcam G8.8 was deposited to the DSHB by Farr, A.G.
F-actin was visualized using Rhodamine phalloidin (Thermo-Fisher, Carlsbad, CA, USA) or Actistain 670 (Cytoskeleton, Denver, CO, USA). Fluorescently conjugated secondary antibodies used were: Dylight 488, 550 and 633 conjugated anti-mouse and anti-rabbit were purchased from Thermo-Fisher. FITC anti-rat, Alexa-fluor 488 anti-mouse, HRP-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch. The polydimethylsiloxane (PDMS) substrates were prepared as previously described(52 (link)).
F-actin was visualized using Rhodamine phalloidin (Thermo-Fisher, Carlsbad, CA, USA) or Actistain 670 (Cytoskeleton, Denver, CO, USA). Fluorescently conjugated secondary antibodies used were: Dylight 488, 550 and 633 conjugated anti-mouse and anti-rabbit were purchased from Thermo-Fisher. FITC anti-rat, Alexa-fluor 488 anti-mouse, HRP-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch. The polydimethylsiloxane (PDMS) substrates were prepared as previously described(52 (link)).