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Leptin elisa kit

Manufactured by Crystal Chem
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The Leptin ELISA kit is a laboratory assay used to quantitatively measure the levels of the hormone leptin in biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify leptin concentrations.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (1 mentions) Insulin elisa kit, by Crystal Chem (1 mentions) Adiponectin elisa kit, by Crystal Chem (1 mentions) Edta free protease inhibitor cocktail, by Roche (1 mentions) Free fatty acid quantification kit, by Merck Group (1 mentions) Igf 1 elisa kit, by R&D Systems (1 mentions) Adiponectin elisa kit, by ALPCO (1 mentions) Somatostatin elisa kit, by Phoenix Pharmaceuticals (1 mentions)

Close spelling variants of this product

Mouse Leptin ELISA Kit (65 citations) Leptin (27 citations) Rat Leptin ELISA kit (6 citations) ELISAs (3 citations) Insulin and leptin ELISA kits (2 citations)

5 protocols using leptin elisa kit

1

Mouse Plasma Leptin Quantification

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Leptin ELISA kit was from Crystal Chem (Cat# 90030). Leptin concentration was measured using mouse plasma according to manufacturer’s instructions. Blood was collected in heparinized vials, and centrifuged for 60min at 3000rpm at 4°C. Plasma was collected and stored in −80 °C until further processing.
Çakır I., Hadley C.K., Pan P.L., Bagchi R.A., Ghamari-Langroudi M., Porter D.T., Wang Q., Litt M.J., Jana S., Hagen S., Lee P., White A., Lin J.D., McKinsey T.A, & Cone R.D. (2022). Histone Deacetylase 6 Inhibition Restores Leptin Sensitivity and Reduces Obesity. Nature metabolism, 4(1), 44-59.
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2

Serum Leptin Quantification Protocol

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Serum leptin levels were determined using a leptin ELISA kit (Crystal Chem Inc., Chicago, IL, USA) according to the manufacturer’s instructions.
Hoek-van den Hil E.F., van Schothorst E.M., van der Stelt I., Swarts H.J., van Vliet M., Amolo T., Vervoort J.J., Venema D., Hollman P.C., Rietjens I.M, & Keijer J. (2015). Direct comparison of metabolic health effects of the flavonoids quercetin, hesperetin, epicatechin, apigenin and anthocyanins in high-fat-diet-fed mice. Genes & Nutrition, 10(4), 23.
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3

Metabolic Biomarker Profiling in Mice

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Serum insulin, ghrelin, adiponectin, leptin and free fatty acid were measured with ultrasensitive mouse Insulin ELISA kits (Crystal Chem, #90080), Ghrelin (total) Elisa kit (#EZRGRT-91K, Millipore), Adiponectin ELISA kit (Crystal Chem, #80569), Leptin ELISA kit (Crystal Chem, #90030), free fatty acid quantification kit (MAK044-1KT, Sigma Aldrich) respectively, according to manufacturers’ instruction.
For p-AKT/Total AKT measurement, inguinal adipose tissue (iWAT) extracts were homogenized and lysed in RIPA buffer (ThermoFisher, #89901) containing protease and phosphatase inhibitors (Roche, cOmplete, EDTA-free Protease Inhibitor Cocktail, PhosSTOP). Total AKT and p-AKT levels in the cell lysates quantified using the p-AKT/Total AKT kit (PEL-AKT-S473-T-1, RayBiotech), as per manufacturer’s instructions.
Marino L., Ni B., Farrar J.S., Lownik J.C., Pearce J.V., Martin R.K, & Celi F.S. (2024). Adipose tissue-selective ablation of ADAM10 results in divergent metabolic phenotypes following long-term dietary manipulation. Adipocyte, 13(1), 2339418.
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4

Mouse Plasma Leptin Quantification

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Leptin ELISA kit was from Crystal Chem (Cat# 90030). Leptin concentration was measured using mouse plasma according to manufacturer’s instructions. Blood was collected in heparinized vials, and centrifuged for 60min at 3000rpm at 4°C. Plasma was collected and stored in −80 °C until further processing.
Çakır I., Hadley C.K., Pan P.L., Bagchi R.A., Ghamari-Langroudi M., Porter D.T., Wang Q., Litt M.J., Jana S., Hagen S., Lee P., White A., Lin J.D., McKinsey T.A, & Cone R.D. (2022). Histone Deacetylase 6 Inhibition Restores Leptin Sensitivity and Reduces Obesity. Nature metabolism, 4(1), 44-59.
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5

Blood Serum Analysis in Fasted Mice

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The mice were fasting 4 hours before collection of the whole blood. The serum was collected by centrifuge at 2,000 x g for 10 minutes at 4°C. The ELISA was preformed according to the manual. The leptin ELISA kit (catalog # 90030) was purchased in Crystal Chem, IGF‐1 ELISA kit (catalog # MG100) was R&D SYSTEM, the high‐molecular‐weight adiponectin ELISA kit (catalog # 47‐ADPMS‐E01) was ALPCO, and somatostatin ELISA kit (EK‐060–03) was phoenix pharmaceuticals.
Icyuz M., Zhang F., Fitch M.P., Joyner M.R., Challa A.K, & Sun L.Y. (2021). Physiological and metabolic characteristics of novel double‐mutant female mice with targeted disruption of both growth hormone‐releasing hormone and growth hormone receptor. Aging Cell, 20(4), e13339.
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