Tufts of aeciospores were picked off by scraping infected leaves with a dissecting needle in correspondence of the aecia under a stereo microscope Nikon SM2800® (Nikon Corporation, Tokyo, Japan). Aeciospores were then transferred to glass slides and mounted in a drop of 0.5% KOH or water for direct examination under the light microscope. Spore morphology and size were determined under an Eclipse 80i microscope (Nikon) dotted with digital sight images (DS-L2 imaging controller, Nikon, Melville, NY, USA).
Ds l2 imaging controller
Manufactured by Nikon
Sourced in United States
The DS-L2 imaging controller is a device designed for microscope camera control and image capture. It allows users to capture, display, and save digital images from Nikon cameras attached to microscopes. The DS-L2 provides basic functionality for camera operation and image management, facilitating the integration of digital imaging capabilities into microscopy workflows.
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Lab products found in correlation
2 protocols using ds l2 imaging controller
1
Microscopic Examination of Aeciospores
2
Toluidine Blue Staining for Mast Cell Identification
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Toluidine blue staining was used to label mast cells and performed as previously reported with minor modifications [21] . Briefly, the subcutaneous connective tissue in contact with the air pouch was isolated, stretched onto a microscope slide, and dried quickly. The tissue was fixed with Carnoy's fixative composed of ethanol, chloroform, and glacial acetic acid (6:3:1) for 5 h. The slides were then immersed sequentially in 100% and 80% ethanol for 1 minute each and fully rinsed in tap water for 5 minutes. The preparations were stained with 0.1% toluidine blue for 30 minutes at room temperature and washed with tap water. For dehydration, the slides were immersed sequentially in 80% and 100% ethanol for 1 minute each, in 100% ethanol for 10 minutes and then in xylene for 10 minutes. The preparations were mounted with Bioleit (Okenshoji Co., Ltd., Tokyo, Japan) and observed using a light microscope (Labophot-2; Nikon, Tokyo, Japan). The images were captured using Nikon Digital Sight DS-Fi1 camera and a DS-L2 imaging controller.
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