2 amino 2 hydroxymethyl propane 1 3 diolhydrochloride

Manufactured by Merck Group

Sourced in Germany

2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride is a chemical compound used in laboratory settings. It functions as a buffer solution and has applications in various biochemical and analytical procedures.

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Lab products found in correlation

4 protocols using 2 amino 2 hydroxymethyl propane 1 3 diolhydrochloride

Lipid Vesicle Preparation and Characterization

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All the solutions were prepared by using dust-free Milli-Q water (γ= 72.8 mN m-1 and resistivity of 18.2 MΩ cm). Sodium dodecylsulfate (SDS), polyoxyethylene-9-lauryl ether (polidocanol, C12(EO)9), potassium bromide, magnesium chloride, sodium chloride, p-nitrophenylphosphate (pNPP), chloroform, 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride (Tris), and 2-amino-2-methyl-propan-1-ol (AMPOL) were purchased from Sigma Chemical Co. [(2R)-2,3-Di(hexadecanoyloxy)propyl] 2-(trimethylazaniumyl)ethyl phosphate (dipalmitoylphosphatidyl-choline, DPPC) (99+%) and (8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl] 1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one (cholestenone, A-Chol) (99+%) were obtained from AVANTI Polar Lipids. Methanol (MeOH, 99+%) was supplied by J.T. Baker. 3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine (acridine orange, AO) was acquired from Acros Organic. TNAP was obtained from osteoblasts primary cultures as previously reported elsewhere [15 (link)]. Calbiosorb was purchased from Merck-Millipore.

Andrade M.A., Favarin B., Derradi R., Bolean M., Simão A.M., Millán J.L., Ciancaglini P, & Ramos A.P. (2016). Pendant-drop method coupled to ultraviolet-visible spectroscopy: A useful tool to investigate interfacial phenomena. Colloids and surfaces. A, Physicochemical and engineering aspects, 504, 305-311.

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Synthesis of Biomolecular Compounds

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Acrylonitrile, 2-Amino-2-hydroxymethyl-propane-1,3-diol hydrochloride (Tris-HCl), and 4-(2-hydroxyethyl)piperazine-1ethanesulfonic acid (HEPES) were obtained from Sigma-Aldrich. All other materials were purchased at the highest quality available.

Martinez S., Wu R., Krzywda K., Opalka V., Chan H., Liu D, & Holz R.C. (2015). Analyzing the catalytic role of active site residues in the Fe-type nitrile hydratase from Comamonas testosteroni Ni1. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 20(5).

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Comprehensive Biological Reagent Procurement

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Trichloroacetic acid, potassium chloride, sucrose, 3-(N-morpholino) propanesulfonic acid (MOPS), iodoacetic acid, ethylenediamine tetra-acetic acid (EDTA), potassium hydroxide, phosphoric acid, acetonitrile HPLC grade, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES), sodium chloride, 2amino2-hydroxymethyl-propane-1,3-diol-hydrochloride (Tris-HCl), sucrose, trypsin, bovine serum albumin (BSA), Trichloroacetic acid, sodium carbonate, and D-mannitol were obtained from Merck (Darmstadt, Germany). Ketamine and xylazine were purchased from Bioveta (Czech Republic). Anhydride calcium, methyl tetrazolium, dinitrofluorobenzene (DNFB), dimethyl sulfoxide, and taurine (2-aminoethanesulfonic acid) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Najibi A., Rezaei H., Manthari R.K., Niknahad H., Jamshidzadeh A., Farshad O., Yan F., Ma Y., Xu D., Tang Z., Ommati M.M, & Heidari R. (2022). Cellular and mitochondrial taurine depletion in bile duct ligated rats: a justification for taurine supplementation in cholestasis/cirrhosis. Clinical and Experimental Hepatology, 8(3), 195-210.

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Evaluating Hepatoprotective Agents: A Protocol

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Taurine (2-amino-ethane sulfonic acid) was obtained from Acros (New Jersey, USA). Acetaminophen, thioacetamide, trichloroacetic acid (TCA), 5,5′-dithionitrobenzoic acid (DTNB), thiobarbituric acid (TBA), sodium citrate, sodium hypochlorite solution (5%), ammonium chloride, nicotinamide adenine dinucleotide phosphate (NADPH), phenol, sodium nitroprusside dihydrate, ethylenediamine tetra-acetic acid (EDTA), 4-nitrophenol, phosphoric acid, 2‐amino‐2-hydroxymethyl-propane-1,3-diol-hydrochloride (Tris–HCl), were obtained from Merck (Darmstadt, Germany). Kits for evaluating biomarkers of liver injury, including ALT, LDH, AST, ALP and bilirubin, were obtained from Pars Azmun^® (Tehran, Iran). All salts used for preparing buffer solutions were of analytical grade and obtained from Merck (Darmstadt, Germany).

Heidari R., Jamshidzadeh A., Niknahad H., Mardani E., Ommati M.M., Azarpira N., Khodaei F., Zarei A., Ayarzadeh M., Mousavi S., Abdoli N., Yeganeh B.S., Saeedi A, & Najibi A. (2016). Effect of taurine on chronic and acute liver injury: Focus on blood and brain ammonia. Toxicology Reports, 3, 870-879.

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