Tamarixetin

All genes, bacterial strains, and plasmids used in this study are listed in Table 1. All CaFLS mutants used in this study are listed in Table 2. The gene and protein sequences used in this study are listed in Supplementary materials S1, S2, respectively. All the wild-type genes and mutanted genes were synthesized and sequenced by Sangon Biotech (Shanghai, China). The restriction nuclease was obtained from Sangon Biotech (Shanghai, China). Hesperetin and diosmetin was procured from Macklin (Shanghai, China) and Sigma-Aldrich (St. Louis, MO, United States). diosmetin, 4′-O-methyl taxifolin, and tamarixetin reference material were purchased from Sigma-Aldrich. Isopropyl-β-D-thiogalactoside (IPTG), Luria-Bertani broth (LB), and kanamycin were purchased from Solarbio (Beijing, China).

Quercetin, quercitrin, isorhamnetin, tamarixetin, L-glutamine, and trypsin were purchased from Sigma (USA). HPLC-grade methanol was obtained from Thermo Fisher (Waltham, MA, USA), Dulbecco’s Modified Eagle’s medium (DMEM) from Gibco (Waltham, MA, USA), and fetal bovine serum and nonessential amino acids from Hyclone (Logan, UT, USA). Ultrapure water was prepared by using a MilliPore ultrapure water purifier (St. Louis, MO, USA). All other chemicals used in the experiments were of analytical grade. The 12-well Millicell plates were purchased from Millipore. LC/MS (Agilent 1100 LC/MS DVL, DE) was obtained from Agilent (Santa Clara, CA, USA).