Serum samples were collected monthly and directly stored at −20°C until after preparation because NfL protein is stable during freezing process.24 (link),25 (link) sNfL measurement was performed using the Advantage NF-Light Singleplex Kit and prepared as defined in the manufacturer's instructions (Quartered, Lexington, MA, Datasheet Quanterix: SIMOA NF-Light Advantage Kit). The SIMOA Human Advantage NfL assay is a digital immunoassay for the quantitative determination of NfL and was ran on a Simoa HD-1 instrument (Quanterix).26 (link) The antibodies and calibrators used in the assays have been developed by Uman Diagnostics (Sweden) and described previously in their plate-based enzyme-linked immunosorbent assay.27 (link) Previous reports proved that the newly developed SIMOA technique is the most sensitive to detect NfL even in lowest concentrations.28 (link) Calibrators (8 calibrator points) and diluted serum samples (dilution 1:4) were measured in duplicates. Sample dilution was calculated using the instrument. The lower threshold of quantification was 0.775 pg/mL. Both the mean intraassay coefficient of variation of duplicates and the mean interassay coefficient of variation were <10%.
Advantage nf light singleplex kit
Manufactured by Quanterix
The Advantage NF-Light Singleplex-Kit is a lab equipment product designed for singleplex protein analysis. It provides a platform for the detection and quantification of individual protein targets in a sample.
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Lab products found in correlation
3 protocols using advantage nf light singleplex kit
1
Quantification of Neurofilament Light Protein
2
Quantification of Serum neurofilament Light
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Serum samples were stored at −20 °C and sNfL measurements were performed as described previously, using the Advantage NF-Light Singleplex-Kit on a Simoa HD-1 instrument (Quanterix)27 (link),47 (link). Calibrators and diluted serum samples were measured in duplicates. Both the mean intraassay coefficient of variation of duplicates and the mean interassay coefficient of variation were <10%.
3
Neurofilament Light Quantification
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Serum samples were stored at − 20 °C after preparation. NFL measurement was performed as described previously19 (link),20 (link), using the Advantage NF-Light Singleplex-Kit on a Simoa HD-1 instrument (Quanterix). Calibrators and diluted serum samples were measured in duplicates. The lower threshold of quantification was 0.775 pg/ml. Both the mean intraassay coefficient of variation of duplicates and the mean interassay coefficient of variation were < 10%.
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