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Purified salmonella typhimurium flagellin

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Purified Salmonella Typhimurium flagellin is a bacterial protein component isolated from the flagella of the Salmonella Typhimurium bacterium. It is a purified, lyophilized powder form of this protein.

Automatically generated - may contain errors

Lab products found in correlation

Tlrl stfla, by InvivoGen (2 mentions) Hkb null1v, by InvivoGen (2 mentions) Hkb mtlr5, by InvivoGen (2 mentions)

Close spelling variants of this product

Salmonella typhimurium flagellin Salmonella typhimurium Purified flagellin Flagellin

2 protocols using purified salmonella typhimurium flagellin

1

SFB Culture Flagellin Bioactivity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Equal numbers of IO-only and filament enriched SFB fractions, based on 16S rDNA qPCR analysis, from 3-day old in vitro-grown SFB cultures were sonicated for 10 min (Bioblock, 88154) and 1/5 dilutions of lysate were added to control and mTLR5-expressing HEK cells in HEK-Blue detection medium (Invivogen, hkb-null1v, hkb-mtlr5, hb-det2) seeded in 96-well plates for 16 hours before alkaline phosphatase activity was measured at 620 nm using an ELISA plate reader (Labsystem Multiscan RC). Purified Salmonella Typhimurium flagellin (Invivogen, tlrl-stfla) served as the positive control.
Nkamba I., Mulet C., Dubey G.P., Gorgette O., Couesnon A., Salles A., Moya-Nilges M., Jung V., Gaboriau-Routhiau V., Guerrera I.C., Shima T., Umesaki Y., Nigro G., Krijnse-Locker J., Bérard M., Cerf-Bensussan N., Sansonetti P.J, & Schnupf P. (2019). Intracellular offsprings released from SFB filaments are flagellated. Nature microbiology, 5(1), 34-39.
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2

SFB Culture Flagellin Bioactivity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Equal numbers of IO-only and filament enriched SFB fractions, based on 16S rDNA qPCR analysis, from 3-day old in vitro-grown SFB cultures were sonicated for 10 min (Bioblock, 88154) and 1/5 dilutions of lysate were added to control and mTLR5-expressing HEK cells in HEK-Blue detection medium (Invivogen, hkb-null1v, hkb-mtlr5, hb-det2) seeded in 96-well plates for 16 hours before alkaline phosphatase activity was measured at 620 nm using an ELISA plate reader (Labsystem Multiscan RC). Purified Salmonella Typhimurium flagellin (Invivogen, tlrl-stfla) served as the positive control.
Nkamba I., Mulet C., Dubey G.P., Gorgette O., Couesnon A., Salles A., Moya-Nilges M., Jung V., Gaboriau-Routhiau V., Guerrera I.C., Shima T., Umesaki Y., Nigro G., Krijnse-Locker J., Bérard M., Cerf-Bensussan N., Sansonetti P.J, & Schnupf P. (2019). Intracellular offsprings released from SFB filaments are flagellated. Nature microbiology, 5(1), 34-39.
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