Fusion fx6 instrument

Manufactured by Thermo Fisher Scientific

The Fusion FX6 instrument is a versatile and powerful electrophoresis system designed for a range of applications, including DNA, RNA, and protein analysis. It features a modular design that allows for customization and flexibility to meet the needs of various research and diagnostic laboratories.

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Lab products found in correlation

Cocktail of protease inhibitor, by Merck Group (2 mentions) Flotillin 1, by Thermo Fisher Scientific (2 mentions) Peif2a, by Thermo Fisher Scientific (2 mentions) Cd63 nvg 2, by BioLegend (2 mentions) Cd81 eat 2, by BioLegend (2 mentions) Cd9 em 04, by Abcam (2 mentions) β actin w16197a, by BioLegend (2 mentions) Ecl west pico femto, by Thermo Fisher Scientific (2 mentions) Peif2a, by Ozyme (2 mentions) Flotillin 1, by Ozyme (2 mentions)

2 protocols using fusion fx6 instrument

Western Blot Analysis of Cell Lysates

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Protein lysats of cells, AB and MV were prepared in RIPA buffer containing a cocktail of protease inhibitors (Sigma). Proteins were denatured in Laemmli buffer and separated by 4-12% gradient SDS-PAGE in non-reducing (tetraspanins CD81, CD63, CD9) or reducing (all other) conditions and transferred to a nitrocellulose membrane (CD63, flotillin-1, peIF2a and CHOP; Fisher Scientific) or PVDF membrane (all other; BIO-RAD, Marnes La Coquette, France). Membranes were incubated with primary antibodies CD63 (NVG-2; 1:1000; BioLegend), CD81 (Eat-2; 1:1000; BioLegend), CD9 (EM-04; 1:1000; Abcam, Cambridge, UK), polyclonal rabbit anti-calnexin antibody (1:1000; Euromedex, Souffelweyersheim, France), β–actin (W16197A; 1:20,000; Biolegend), flotillin-1 (W16108A; 1:1000; Biolegend), peIF2a (D9G8; 1:1000; Ozyme) and CHOP (D46F1; 1:1000; Ozyme) blocked by either TBS 0.05% Tween-20 4% BSA (CD81, peIF2a, CHOP) or TBS 0.05% Tween-20 5% milk (CD9, CD63, calnexin, flotillin-1), followed by incubation with cognate HRP-conjugated secondary antibodies 1:100,000. The signals were detected with enhanced chemiluminescence substrate (ECL West Pico Femto, Fischer Scientific) on a Fusion FX6 instrument (Fisher Scientific).

Giri K.R., de Beaurepaire L., Jegou D., Lavy M., Mosser M., Dupont A., Fleurisson R., Dubreil L., Collot M., Van Endert P., Bach J.M., Mignot G, & Bosch S. (2020). Molecular and Functional Diversity of Distinct Subpopulations of the Stressed Insulin-Secreting Cell's Vesiculome. Frontiers in Immunology, 11, 1814.

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Western Blot Analysis of Tetraspanins

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Protein lysats of cells, AB and MV were prepared in RIPA buffer containing a cocktail of protease inhibitors (Sigma). Proteins were denatured in Laemmli buffer and separated by 4-12% gradient SDS-PAGE in non-reducing (tetraspanins CD81, CD63, CD9) or reducing (all other) conditions and transferred to a nitrocellulose membrane (CD63, flotillin-1, peIF2a and CHOP; Fisher Scientific) or PVDF membrane (all other; BIO-RAD, Marnes La Coquette, France). Membranes were incubated with primary antibodies CD63 (NVG-2; 1:1000; BioLegend), CD81 (Eat-2; 1:1000; BioLegend), CD9 (EM-04; 1:1000; Abcam, Cambridge, UK), polyclonal rabbit anti-calnexin antibody (1:1000; Euromedex, Souffelweyersheim, France), β-actin (W16197A; 1:20,000; Biolegend), flotillin-1 (W16108A; 1:1000; Biolegend), peIF2a (D9G8; 1:1000; Ozyme) and CHOP (D46F1; 1:1000; Ozyme) blocked by either TBS 0.05% Tween-20 4% BSA (CD81, peIF2a, CHOP) or TBS 0.05% Tween-20 5% milk (CD9, CD63, calnexin, flotillin-1), followed by incubation with cognate HRP-conjugated secondary antibodies 1:100,000. The signals were detected with enhanced chemiluminescence substrate (ECL West Pico Femto, Fischer Scientific) on a Fusion FX6 instrument (Fisher Scientific).

Giri K.R., Beaurepaire L.d., Jégou D., Lavy M., Mosser M., Dupont A., Fleurisson R., Dubreil L., Collot M., Endert P.V., Bach J., Mignot G., & Bösch S. (2020). Molecular and functional diversity of distinct subpopulations of extracellular vesicles from stressed pancreatic beta cells: implications for autoimmunity.

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