Antibodies against phospho-p70 S6 kinase (Thr389), p70 S6 kinase, phospho-S6 (Ser235/236), S6, LC3B and α-tubulin were purchased from Cell Signaling Technology (Danvers, MA, USA; cat. nos. 9205, 9202, 4858, 2217, 2775 and 3873). Antibodies against GS were obtained from EMD Millipore (Danvers, MA, USA; cat. no. MAB302). Antibodies against β-actin were purchased from Sigma-Aldrich (St. Louis, MO, USA; cat. no. A5441). Horseradish peroxidase-conjugated anti-mouse immunoglobulin G and horseradish peroxidase-conjugated anti-rabbit immunoglobulin G were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA; cat. nos. sc-2748 and sc-2749).
Horseradish peroxidase conjugated anti mouse immunoglobulin g
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Horseradish peroxidase-conjugated anti-mouse immunoglobulin G is a laboratory reagent used in various immunoassay techniques. It consists of anti-mouse IgG antibodies conjugated to the enzyme horseradish peroxidase. This conjugate can be used to detect and quantify mouse immunoglobulin G in biological samples.
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2 protocols using horseradish peroxidase conjugated anti mouse immunoglobulin g
1
Western Blot Antibody Reagents
2
Western Blot Protein Detection
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The purified target protein was run on 10% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. The membranes were then blocked with PBS containing 0.05% Tween-20 and 5% dry milk. The bound proteins were detected using mouse antiserum (diluted 1:12,000), and incubated for 1h at 37 o C. After washing, horseradish peroxidase-conjugated anti-mouse immunoglobulin G (diluted 1:3000; Santa Cruz Biotech, CA, USA) was added as a secondary antibody and incubated at 37 o C for 2h and washed three times with PBST. The membranes were visualized using o-phenylenediamine dihydrochloride (Sigma-Aldrich, St. Louis, MO, USA) and hydrogen peroxide reagent.
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