Igg rabbit

Manufactured by Abcam

Sourced in United Kingdom

IgG rabbit is a type of immunoglobulin G (IgG) antibody isolated from rabbit serum. IgG antibodies are the most abundant antibody isotype found in the blood and play a crucial role in the adaptive immune response.

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Lab products found in correlation

Irdye 800cw goat anti rabbit, by LI COR (2 mentions) Igg mouse, by Santa Cruz Biotechnology (1 mentions) Mouse anti pcna, by Abcam (1 mentions) Goat anti lamin b, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti ubf1, by Santa Cruz Biotechnology (1 mentions) Mouse anti ranbp2, by Santa Cruz Biotechnology (1 mentions) Mouse anti coilin, by Merck Group (1 mentions) Mouse anti xpo5, by Abcam (1 mentions) Mouse anti nfkb, by Santa Cruz Biotechnology (1 mentions) Mouse anti fibrillarin, by Abcam (1 mentions) Rabbit anti dyskerin, by Santa Cruz Biotechnology (1 mentions) Anti actin hrp, by Santa Cruz Biotechnology (1 mentions) Rabbit anti h3, by Abcam (1 mentions) Tbs blocking buffer, by LI COR (1 mentions)

Close spelling variants of this product

DyLight 488-conjugated anti-rabbit IgG antibodies FITC-labeled goat anti-rabbit IgG (ab6717, Goat α-rabbit IgG HRP HRP-conjugated goat anti-rabbit IgG Horseradish peroxidase (HRP)–conjugated goat anti-rabbit immunoglobulin G (IgG) secondary antibody Alexa Fluor® 488- or 594-conjugated goat anti-rabbit or anti-mouse IgG Alexa Fluor 594-conjugated anti-rabbit IgG antibody Biotinylated anti-rabbit IgG Goat anti-rabbit IgG H&L (HRP) kit Goat anti-rabbit IgG secondary antibody with Alexa Fluor 594 conjugate

3 protocols using igg rabbit

Antibody Characterization for Cell Biology

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The following are other antibodies used in this study: anti-actin-HRP (horseradish peroxidase) (Santa Cruz, sc-47778), mouse anti-PCNA (Abcam, ab29), rabbit anti-XPO1-C-terminal (Santa Cruz, sc-5595), mouse anti-XPO1-N-terminal (Santa Cruz, sc-136220), mouse anti-Coilin (Sigma C-1862), mouse anti-XPO5 (Abcam ab57491), mouse anti-XPOT (Abcam ab49933), rabbit anti-H3 (Abcam ab1791), mouse anti-GEMIN5 (Santa Cruz, sc-136200), mouse anti-SMN1 (Abcam, ab5831), goat anti-LaminB (Santa Cruz, sc-6216), mouse anti-CSE1L (Santa Cruz sc-135855), rabbit anti-Dyskerin (Santa Cruz sc-48794), rabbit anti-PHAX (Bethyl, A303–916A), goat anti-PHAX (M-19) (Santa Cruz, sc-11704), mouse anti-UBF1 (Santa Cruz sc-13125), mouse anti-nucleolin (Enzo, ADI-KAM-CP100) mouse anti-TRF2 (Imgenex, IG124A), mouse anti-XPO5 (Abcam ab57491), mouse anti-NFKB (Santa Cruz sc-372), mouse anti-RANBP2 (Santa Cruz, sc-74518), mouse anti-Fibrillarin (Abcam, ab4566), mouse anti-FLAG M2 (Sigma), IgG mouse (Santa Cruz sc-2025), mouse anti-Coilin (Sigma, C1862), rabbit anti-TCAB1 (Novus, NB100–68252); IgG rabbit (Abcam ab37415). All Alexa-conjugated secondary antibodies were purchased from Life Technologies.

Schertzer M., Jouravleva K., Perderiset M., Dingli F., Loew D., Le Guen T., Bardoni B., de Villartay J.P., Revy P, & Londoño-Vallejo A. (2015). Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Research, 43(3), 1834-1847.

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Protein Expression Analysis of FKRP in Larval Tissues

Cited 1 time

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Larval heads were removed and used for genotyping, bodies were snap-frozen, homogenised in immunoprecipitation buffer (Pierce). In all, 30 μg of protein was loaded on a 4–12% Bis-Tris Protein Gel MES Buffer (ThermoFisher Scientific). Primary antibodies were prepared in TBS blocking buffer (LI-COR): fkrp: rabbit polyclonal 1:500 fkrp^{48 (link)}, 1:500, Beta-Tubulin-Loading Control (Abcam: ab6046), Golgi 50 kda, IgG rabbit (Abcam: ab6046) 1:10,000, incubated in secondary antibody IRDye 800CW goat anti-rabbit (LI-COR) 1:5000. Blots were scanned on a infrared scanner (Odyssey). Co-immunoprecipitation utilised 10ug of antibody, fibronectin or FKRP, and a protein-G dynabead kit (Thermofisher).

Wood A.J., Lin C.H., Li M., Nishtala K., Alaei S., Rossello F., Sonntag C., Hersey L., Miles L.B., Krisp C., Dudczig S., Fulcher A.J., Gibertini S., Conroy P.J., Siegel A., Mora M., Jusuf P., Packer N.H, & Currie P.D. (2021). FKRP-dependent glycosylation of fibronectin regulates muscle pathology in muscular dystrophy. Nature Communications, 12, 2951.

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Collagen 1a Protein Quantification

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Larvae were homogenized in immunoprecipitation buffer (Pierce, Rockford, Illinois), and 30 μg of protein with was loaded on a 3-8% Tris-Acetate Protein Gel (ThermoFisher Scientific, Waltham, Massachusetts). Primary antibodies were made up in same Licor TBS blocking buffer: Collagen 1a, IgG rabbit (AbCam, United Kingdom: ab2370) 1/500, Beta-Tubulin Loading Control 50 kDa, IgG rabbit (AbCam, United Kingdom: ab6046) 1/10 000 and incubated in secondary antibody IRDye 800CW goat anti rabbit (LI-COR) 1/5000. Blots were scanned on the Odyssey scanner.

Wood A.J., Cohen N., Joshi V., Li M., Costin A., Hersey L., McKaige E.A., Manneken J.D., Sonntag C., Miles L.B., Siegel A, & Currie P.D. (2019). RGD inhibition of itgb1 ameliorates laminin-α2-deficient zebrafish fibre pathology. Human molecular genetics, 28(9).

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