Total RNA was reverse transcribed using SuperScript II Reverse Transcriptase (LifeTechnologies) according to the manufacturer’s protocol. Quantitative PCR was performed with iTaqTM universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad). Hprt, Rpl13 and Sdha were used as normalization controls (Vandesompele et al., 2002 (link)). Each biological replicate was measured in technical duplicates. The primers used for RT-qPCR are listed in Supplementary Table S3 .
Cfx384 c1000 touch
Manufactured by Bio-Rad
The CFX384 C1000 Touch is a real-time PCR detection system manufactured by Bio-Rad. It features a 384-well thermal cycler with a touch screen interface, designed for high-throughput DNA amplification and detection.
Automatically generated - may contain errors
4 protocols using cfx384 c1000 touch
1
Quantitative RT-PCR for Gene Expression
2
Quantitative Real-Time PCR Analysis
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Total RNAs were obtained from cells or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocol. Quantitative PCR were performed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad). Hprt, Ppia and Rpl13 were used as normalization controls 32 (link) .
preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted January 18, 2022. ; https://doi.org/10.1101/2022.01.17.475784 doi: bioRxiv preprint 16 Each biological replicate was measured in technical duplicates. The primers used for qRT-PCR are listed in Supplementary Table 1.
Heatmap was generated using R v4.1.0 and the R package pheatmap v1.0.12 (note: heatmaps displays Z-scores computed on the expression levels of the identified cytokines, measured by qPCR).
preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted January 18, 2022. ; https://doi.org/10.1101/2022.01.17.475784 doi: bioRxiv preprint 16 Each biological replicate was measured in technical duplicates. The primers used for qRT-PCR are listed in Supplementary Table 1.
Heatmap was generated using R v4.1.0 and the R package pheatmap v1.0.12 (note: heatmaps displays Z-scores computed on the expression levels of the identified cytokines, measured by qPCR).
3
Quantitative PCR for Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNAs were obtained from human UREC or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using SuperScript II Reverse Transcriptase (Life Technologies) or High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocol. Quantitative PCR were performed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad). Hprt, Ppia, Rpl13, Sdha and Tbp were used as normalization controls 23 . Each biological replicate was measured in technical duplicates. The primers used for qRT-PCR are listed in Supplementary Table 2.
4
Quantitative PCR for Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNAs were obtained from human UREC or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using SuperScript II Reverse Transcriptase (Life Technologies) or High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocol. Quantitative PCR were performed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad) on a CFX384 C1000 Touch (Bio-Rad). Hprt, Ppia, Rpl13, Sdha and Tbp were used as normalization controls 23 . Each biological replicate was measured in technical duplicates. The primers used for qRT-PCR are listed in Supplementary Table 2.
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