The synthesis of β-sitosterol-β-d -glucoside and β-sitosterol-β-d -galactoside was performed following a procedure described by Kunz and Harreus (61 ), starting from acetobromo-α-d -glucose or acetobromo-α-d -galactose and sitosterol. The remaining compounds were purchased from commercial sources: β-sitosterol (Honeywell-Fluka via Fisher Scientific, Schwerte, Germany), stigmasterol (Acros Organics, Geel, Belgium), stigmasterol-β-d -glucoside (ChemFaces, Wuhan, Hubei, China), and cholesterol-β-d -glucoside and stevioside (both from Sigma-Aldrich, Taufkirchen, Germany). Food medium was then prepared as described above, at the concentrations mentioned in Results.
β sitosterol
Manufactured by Thermo Fisher Scientific
Sourced in Germany
β-sitosterol is a plant-derived sterol compound that is commonly used as a reference standard or analytical marker in various laboratory applications. It is a naturally occurring sterol found in many plants, including vegetables, fruits, and nuts. β-sitosterol is often used in analytical methods to identify and quantify the presence of plant-based sterols in samples.
Automatically generated - may contain errors
Lab products found in correlation
Stevioside, by Merck Group (1 mentions)
Campesterol, by Merck Group (1 mentions)
Ammonium sulfate, by Thermo Fisher Scientific (1 mentions)
Hygromycin b, by Thermo Fisher Scientific (1 mentions)
Yeast nitrogen base, by BD (1 mentions)
Complex yeast extract peptone dextrose ypd, by BD (1 mentions)
Ergosterol, by Merck Group (1 mentions)
2 protocols using β sitosterol
1
Synthesis of Phytosterol Glucosides
2
Engineered Yeast Strains for Sterols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals: campesterol (~98%) and ergosterol (≥75%) are obtained from Sigma-Aldrich. β-Sitosterol (~65%) and stigmasterol (~95%) are purchased from Fisher Scientific. All engineered yeast strains in this work are listed in Table S1 and constructed in a haploid CENPK2.1D background (MATα; his3D;1 leu2-3_112; ura3-52; trp1-289; MAL2-8c; SUC2). Yeast strains were cultured at 30 o C in complex yeast extract peptone dextrose (YPD, all components from BD Diagnostics) medium or synthetic defined medium (SDM) containing yeast nitrogen base (YNB) (BD Diagnostics), ammonium sulfate (Fisher Scientific), 2% (w/v) glucose unless specified and the appropriate dropout (Takara Bio) solution for selection. 200 mg/L G418 sulfate (Calbiochem) or 200 mg/L Hygromycin B (Life Technologies) were used in YPD medium for selection. Plasmids used in this study are in Table S5. Sequences of genes used in this work are listed in Table S6.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!