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Ix71 22fl ph fluorescence microscope

Manufactured by Olympus
Sourced in Japan

The IX71-22FL/PH is a fluorescence microscope manufactured by Olympus. It is designed for viewing and imaging of fluorescently labeled samples. The microscope is equipped with a 22mm field of view and phase contrast capabilities.

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2 protocols using ix71 22fl ph fluorescence microscope

1

Quantifying Cell Viability Assay

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The cells were inoculated into 24-well plates (3 × 10 4 cells/well). After the cells are attached and treated with GD55 and GD55-LHPP at the indicated MOI for 72 h. Subsequently, the cells were incubated in Hoechst 33342 solution (Beyotime, Shanghai, China) for 10 min and then washed twice with PBS. The cells were then observed under the IX71-22FL/PH fluorescence microscope (Olympus Corporation, Japan) at 200 × magnification.
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2

Fluorescence Microscopy of CD55 Signaling

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A total of 5 × 104 cells were inoculated into each well of a 24-well plate, cultured for 12 h, and treated with CD55-EGFP and CD55-Smad4 for an additional 48 h. Subsequently, the cells were incubated in Hoechst 33342 (Beyotime, Shanghai, China) for 10 min and washed twice with PBS. The cells were then observed under the IX71-22FL/PH fluorescence microscope (Olympus Corporation, Japan) at 200× magnification.
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