The active ingredients of TJCYR were examined using the 1200 series HPLC device (Agilent Technologies, Santa Clara, CA, USA) with an autosampler (G1329B), thermostatted column compartment (G1316A), quaternary pump (G1311A), photodiode array detector (G1315D), and degasser (G1322A). HPLC was performed on an Apollo C18 (4.6 × 250 mm; particle size, 5 μm; GRACE, Columbia, Maryland, USA) with a mobile phase of acetonitrile (A) -0.1% (v/v) and phosphoric acid (B) for gradient elution (0–70 min, 1-40% A; 70-90 min, 40-80% A). The detection wavelength was 260 nm, and the flow rate was 1 mL/min. The column temperature was 30°C, and the injection volume was 10 μL. The standard solutions of Calycosin-7-glucoside, Acteoside, Salvianolic acid B, Icariin, Tanshinone IIA, and sample were filtered with a 0.45 μm membrane filter before subjecting them to HPLC analysis.
Apollo c18
Manufactured by Grace Bio-Labs
Sourced in United States
The Apollo C18 is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a C18 stationary phase, which is commonly used for the separation of non-polar and moderately polar analytes. The column provides efficient and reproducible chromatographic separations, making it a versatile tool for various analytical applications.
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2 protocols using apollo c18
1
HPLC Analysis of TJCYR Constituents
2
HPLC Analysis of Phytochemical Compounds
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The phytochemical property of TJCYR was examined by HPLC analysis, comparing it to known compounds that included Calycosin-7-glucoside, Acteoside, Salvianolic acid B, Icariin, Tanshinone IIA. The multi-components of the TJCYR were performed on the 1200 series HPLC device (Agilent Technologies, Santa Clara, CA, USA) equipped with an autosampler (G1329B), thermostatted column compartment (G1316A), quaternary pump (G1311A), photodiode array detector (G1315D), and degasser (G1322A). HPLC was performed on an Apollo C18 (4.6×250mm;partical size, 5μm; GRACE, Columbia, Maryland, USA) with a mobile phase of acetonitrile (A) -0.1% (v/v) phosphoric acid (B) for gradient elution (0-70 min, 1-40% A; 70-90 min, 40-80% A).
The analysis was operated at a flow rate of 1 mL/min, a column temperature of 30 ℃ and a detection wavelength of 260nm. The injection volume was 10μL. The standard solutions and sample were filtered with a 0.45μm membrane filter before subjecting them to HPLC analysis.
The analysis was operated at a flow rate of 1 mL/min, a column temperature of 30 ℃ and a detection wavelength of 260nm. The injection volume was 10μL. The standard solutions and sample were filtered with a 0.45μm membrane filter before subjecting them to HPLC analysis.
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