Clear native-PAGE was performed using 4–16% Bis-tris gels (Novex, Life Technologies), and NativeMARK unstained ladder was used as size standards (Thermo Fisher Scientific). Prior to loading the gel, FopA was mixed with native-PAGE Sample Buffer (100 mM sodium chloride, 100 mM imidazole-HCl, 4 mM 6-aminohexanoic acid, 10% glycerol, 2 mM EDTA, pH 7.0) in the volume ratio of 1:2. Electrophoresis was performed at 150 V, 10 mA using Anode Buffer (25 mM imidazole, pH 7.0) and Cathode Buffer (0.05% sodium deoxycholate, 0.01% βDDM, 50 mM Tricine, 7.5 mM imidazole, pH 7.0) in the electrophoresis chamber system (Invitrogen, Life Technologies) at 4°C.
Nativemark unstained ladder
Manufactured by Thermo Fisher Scientific
The NativeMARK unstained ladder is a molecular weight standard used for the analysis of proteins in native polyacrylamide gel electrophoresis (native PAGE) applications. It provides a reference for estimating the molecular weights of proteins under non-denaturing conditions.
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2 protocols using nativemark unstained ladder
1
Native-PAGE Analysis of FopA Protein
2
Native-PAGE Analysis of FopA Protein
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Clear native-PAGE was performed using 4 -16 % Bis-tris gels (Novex, Life Technologies), and NativeMARK unstained ladder was used as size standards (Thermo Fisher Scientific). Prior to loading the gel, FopA was mixed with native-PAGE Sample Buffer (100 mM sodium chloride, 100 mM imidazole-HCl, 4 mM 6-aminohexanoic acid, 10% glycerol, 2 mM EDTA, pH 7.0) in the volume ratio of 1:2. Electrophoresis was performed at 150 V, 10 mA using Anode Buffer (25 mM imidazole, pH 7.0) and Cathode Buffer (0.05% sodium deoxycholate, 0.01% βDDM, 50 mM Tricine, 7.5 mM imidazole, pH 7.0) in the electrophoresis chamber system (Invitrogen, Life Technologies) at 4°C.
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