Drop out mix for yeast synthetic medium

Manufactured by US Biological

Drop Out Mix for yeast synthetic medium is a pre-mixed powder formulation that provides amino acids, vitamins, and other nutrients required for the growth of yeast strains in defined synthetic media. The specific components and their concentrations are designed to support the optimal growth of genetically engineered yeast cells.

Automatically generated - may contain errors

Lab products found in correlation

5 foa, by Cayman Chemical (2 mentions) Dynabeads myone streptavidin c1, by Thermo Fisher Scientific (1 mentions) H3k56ac, by Active Motif (1 mentions) C myc 9e10, by Merck Group (1 mentions) Ecl solution, by Promega (1 mentions) Probequant g 50 micro column, by GE Healthcare (1 mentions) G6pdh, by Merck Group (1 mentions) Dyskerin, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) T4 polynucleotide kinase, by New England Biolabs (1 mentions) γ 32p atp, by PerkinElmer (1 mentions) Protein g coupled dynabeads, by Thermo Fisher Scientific (1 mentions) Thiolutin, by Abcam (1 mentions) β actin gtx109639, by GeneTex (1 mentions) Secondary antibodies against rabbit 7074s and mouse 7076s igg, by Cell Signaling Technology (1 mentions) Iaid tag m214 3, by MBL Life Science (1 mentions) Ampure xp bead, by Beckman Coulter (1 mentions)

2 protocols using drop out mix for yeast synthetic medium

Yeast Strain and Plasmid Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genotypes of yeast strains and plasmids are listed in Appendix Tables S2 and S3. All chemicals used in this study were purchased analytical grade from either Sigma‐Aldrich or Carl Roth, unless stated otherwise. Drop Out Mix for yeast synthetic medium was from US Biological Life Sciences (D9543‐01). 5‐FOA was bought from Cayman Chemicals (17318). Protein‐G coupled Dynabeads™ (10004D) and streptavidin‐coated Dynabeads™ MyOne™ Streptavidin C1 (65001) were from Thermo Fisher. Secondary antibodies against rabbit (7074S) and mouse IgG (7076S) coupled to HRP were purchased from Cell Signaling. Primary antibodies were as follows: H3K56ac (Active Motif – 39281), G6PDH (Sigma – A9521) and c‐Myc (9e10, Sigma – MABE282), FLAG (Sigma – F3165), H2AQ105me from (Tessarz et al, 2014 (link)), H2A (Abcam – ab13923), H3 (Abcam – ab1791), Utp11L (GeneTex GTX115929), Dyskerin (Santa Cruz sc‐373956) and human Nhp2 (Proteintech 15128‐1‐AP). ECL solution was from Promega (W1001). T4 Polynucleotide kinase was from NEB (M0201) and Probe Quant G‐50 microcolumns from GE Healthcare (28‐9034‐08). γ‐(³²P)‐ATP was purchased from Perkin Elmer (BLU002Z250UC).

Mawer J.S., Massen J., Reichert C., Grabenhorst N., Mylonas C, & Tessarz P. (2021). Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis. EMBO Reports, 22(10), e52435.

+ Open protocol

+ Expand

Yeast Genetic Manipulation Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genotypes of strains and yeast plasmids used in this work are listed in Supplementary Table 3 and4. All chemicals used in this study were purchased analytical grade from either Sigma Aldrich or Carl Roth except for the following: Drop Out Mix for yeast synthetic medium was from US Biological Life Sciences (D9543-01). 5-FOA was bought from Cayman Chemical (17318). Ampure XP beads were from Beckman Coulter (A63881), Protein-G coupled dynabeads from Thermo Fisher (10009D) and Thiolutin from Abcam (ab143556). Secondary antibodies against rabbit (7074S) and mouse (7076S) IgG coupled to HRP were purchased from Cell Signaling. Antibodies against H3K56ac were from Active Motif (39281), G6PDH (A9521) and c-Myc (MABE282) from Sigma, iAID-tag (M214-3) from MBL International and βactin (GTX109639) from GeneTex.

Kochan D.Z., Mawer J.S.P., Tishinov K., Parekh S., Massen J., Graef M., Spang A., & Tessarz P. (2020). The RNA-binding protein Puf5 buffers mRNA levels against chromatin-mediated changes in nascent transcription.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!