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2 protocols using 3h dihydrotestosterone

1

Steroid Hormone Measurement Protocol

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[3H]Pregnenolone, [3H]progesterone, [3H]androstenedione, [3H]testosterone, and [3H]dihydrotestosterone were purchased from DuPont-New England Nuclear (Boston, MA). Unlabeled pregnenolone, progesterone, 17α-hydroxyprogesterone, androstenedione, and testosterone were obtained from Steraloids (Newport, RI). Dibutyl phthalate and monobutyl phthalate were purchased from Sigma (St. Louis, MO). Male Sprague Dawley rats (30-day-old) were purchased from Shanghai Animal Center (Shanghai, China). All animal procedures were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University and were performed in accordance with the Guide for the Care and Use of Laboratory Animals.
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2

Steroid Metabolism in Rats

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[3H]Pregnenolone, [3H]progesterone, [3H]androstenedione, [3H]testosterone, [3H]dihydrotestosterone were purchased from DuPont-New England Nuclear (Boston, MA). Unlabeled pregnenolone, progesterone, 17α-hydroxyprogesterone, androstenedione and testosterone were obtained from Steraloids (Newport, RI). Etomidate was purchased from Sigma (St. Louis, MO). Male Sprague-Dawley rats (30-day-old) were purchased from Shanghai Animal Center (Shanghai, China). All animal procedures were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University and were performed in accordance with the Guide for the Care and Use of Laboratory Animals.
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