All animal procedures were conducted in accordance with protocols approved by the Illinois Institutional Animal Care and Use Committee at the University of Illinois at Urbana-Champaign. All experiments in this study were carried out in compliance with the ARRIVE guidelines. Approximately 9 × 106 MAT B III rat mammary adenocarcinoma cells (CRL-1666, American Type Culture Collection) were grown in the same media comprised of McCoy’s 5A (Modified) medium (16600082, Thermo Fisher Scientific) supplemented with 10% v/v fetal bovine serum (16140071, Thermo Fisher Scientific, Waltham, MA, USA) and 1% v/v Penicillin–Streptomycin (10378016, Thermo Fisher Scientific), and were injected subcutaneously into a healthy rat. After 7 days, when the tumor was approximately 5 × 5 × 2 mm3, the rat was euthanized by isoflurane overdose. Approximately 15 min postmortem, the tumor was surgically resected and placed in an imaging dish containing cold saline.
Crl 1666
Manufactured by American Type Culture Collection
Sourced in United States
The CRL-1666 is a cell line derived from human adipose tissue. It is a fibroblast-like cell type that can be used for in vitro studies.
Automatically generated - may contain errors
2 protocols using crl 1666
1
Rat Mammary Adenocarcinoma Tumor Resection
2
Culturing Rat Mammary Adenocarcinoma Cells
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Rat mammary adenocarcinoma (CRL-1666) was selected and purchased from the American Type Culture Collection. The cells, which were initially established at the EG&G Mason Research Institute, were cultured in Dulbecco Modified Eagle Medium containing 10% fetal bovine serum, 80.5 pg/mL of streptomycin, and maintained in a humidified atmosphere of 5% CO2 at 37°C.
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