PC cell lines were purchased from American Type Culture Collection (ATCC). The human pancreatic ductal epithelial cell (HPDE), ASPC, and BXPC-3 cells were cultured in RPMI 1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Gibco, USA). PANC-1, MIA PaCa-2, and SW1990 cells were cultured in DMEM medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Gibco, USA). All cells were cultured in 5% CO2 at 37 °C. The LINC00857 overexpression lentiviral vector (LINC00857), LINC00857 shRNA lentiviral vectors (sh-LINC00857#1 and LINC00857#2), the corresponding negative control lentiviral vectors (NC and sh-NC), miR-130b mimics, control mimic, miR-130b inhibitors, and control inhibitor were purchased from Ribobio (Guangzhou China). The RHOA overexpression lentiviral vector (RHOA), negative control lentivirus (NC), and small interfering RHOA (si-RHOA) were designed by Shanghai Jikai Biological Co., Ltd. All process reference reagent manufacturers’ instructions for transfection of PC cells PANC-1 and BXPC-3.
Pc cell lines
Manufactured by American Type Culture Collection
Sourced in United States
PC cell lines are a collection of immortalized cell lines derived from various tissues and organisms. These cell lines are maintained and distributed by the American Type Culture Collection (ATCC) for use in scientific research and applications. PC cell lines are characterized by their ability to proliferate indefinitely in culture, making them a valuable tool for researchers studying a wide range of topics, including cell biology, genetics, and disease modeling.
Automatically generated - may contain errors
2 protocols using pc cell lines
1
Overexpression and Knockdown of LINC00857 in Pancreatic Cancer Cells
2
Human Pancreatic Cell Culture
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Human pancreatic duct epithelial (HPDE) cells and PC cell lines were obtained from the American Type Culture Collection and cultured in Dulbecco’s minimal essential medium (DMEM) or Roswell Park Memorial Institute (RPMI)-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc., CA, USA) containing 10% fetal bovine serum (FBS) (Invitrogen; Thermo Fisher Scientific, Inc.) and penicillin/streptomycin in a humidified 37 ℃, 5% CO2 incubator. Oligonucleotides used for this study were obtained from Ribobio (Guangzhou, China).
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