Human U87, U373, and LN229 glioblastoma cells were obtained from the American Type Culture Collection (ATCC). The cells were cultured in DMEM supplemented with 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin at 37°C with 5% CO2 in a humidified atmosphere. Brevilin A was dissolved in DMSO to obtain a 5-mM stock solution and kept at 4°C in the dark, protected from light. Cells were treated with Brevilin A dissolved in DMSO, with a final DMSO concentration of 0.5% which was found to be nontoxic as determined by our pilot experiment. Control cells were treated with 0.5% DMSO.
Ln229 glioblastoma cells
Manufactured by American Type Culture Collection
The LN229 glioblastoma cells are a well-characterized in vitro model derived from a human glioblastoma multiforme (GBM) tumor. These cells exhibit typical glioblastoma features and are commonly used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ln229, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
Pyruvate, by Thermo Fisher Scientific (1 mentions)
Hek293ft, by Thermo Fisher Scientific (1 mentions)
Hek293ft cells, by Thermo Fisher Scientific (1 mentions)
2 hydroxyglutarate, by Merck Group (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
2 protocols using ln229 glioblastoma cells
1
Glioblastoma Cell Culture and Brevilin A Treatment
2
Glioma Tissue and Cell Culture Analysis
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Analyses of patient material were performed in accordance with the guidelines of the local medical ethical committee of the Radboud University Medical Center, Nijmegen, The Netherlands. Patient glioma samples (n = 83), comprising 19 grade II, 15 grade III and 49 grade IV tumors, were obtained from the archives of the Radboud University Medical Center [see Additional file 1 ]. Histologically normal brain control tissue (temporal neocortex and white matter) was obtained from surgery on epilepsy patients at the VU University Medical Center, Amsterdam, The Netherlands.
HEK293FT cells were purchased from Invitrogen, LN-229 glioblastoma cells were from the American Type Culture Collection, and U-251 MG cells were kindly provided by Joost Schalkwijk (Radboudumc). Derivation and use of the xenograft-derived glioblastoma cell model E98 has been described elsewhere [21 (link)]. U-251 MG, LN-229, HEK293FT and E98 cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (Life Technologies; cat.no.11960–044) supplemented with 10 % Fetal Bovine Serum (PAA laboratories; Cat.no. A15–101), 4 mM glutamine and 1 mM pyruvate (Life Technologies), at 37 °C in a humidified incubator under 7.5 % CO2. For some experiments 1 μM Gefitinib (Selleck Chemicals), 4 mM 2-hydroxyglutarate (Sigma H8378), or DMSO (solvent control) was added 48 h prior to isolation.
HEK293FT cells were purchased from Invitrogen, LN-229 glioblastoma cells were from the American Type Culture Collection, and U-251 MG cells were kindly provided by Joost Schalkwijk (Radboudumc). Derivation and use of the xenograft-derived glioblastoma cell model E98 has been described elsewhere [21 (link)]. U-251 MG, LN-229, HEK293FT and E98 cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (Life Technologies; cat.no.11960–044) supplemented with 10 % Fetal Bovine Serum (PAA laboratories; Cat.no. A15–101), 4 mM glutamine and 1 mM pyruvate (Life Technologies), at 37 °C in a humidified incubator under 7.5 % CO2. For some experiments 1 μM Gefitinib (Selleck Chemicals), 4 mM 2-hydroxyglutarate (Sigma H8378), or DMSO (solvent control) was added 48 h prior to isolation.
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