Axin2lacz

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

Axin2LacZ is a laboratory product that serves as a reporter gene construct. It consists of the Axin2 promoter region fused to the LacZ gene, which encodes the enzyme beta-galactosidase. This construct can be used to monitor the activity of the Axin2 gene, which is involved in the Wnt signaling pathway.

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Lab products found in correlation

10 protocols using axin2lacz

Generating Genetically Modified Mouse Models

Cited 1 time

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Dhcr7^−/− mice^{85 (link)} were a gift from Dr. Forbes D. Porter (The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA). Insig1^F/F;Insig2^−/− (The Jackson Laboratory, #005939)^{12 (link)} and Wnt1-Cre2 (The Jackson Laboratory, #022501)^{22 (link)} mice were obtained from The Jackson Laboratory and crossed to generate Insig1/2 cKO mice. Gli1-LacZ mice (The Jackson Laboratory, #008211)^{39 (link)} were obtained from The Jackson Laboratory and crossed with Dhcr7^+/− and Wnt1-Cre2;Insig1^F/+;Insig2^−/− mice in order to generate Dhcr7^−/−;Gli1-LacZ, Dhcr7^+/+;Gli1-LacZ, Insig1/2 cKO;Gli1-LacZ, and Insig1^F/F;Insig2^−/−;Gli1-LacZ mice. Topgal (The Jackson Laboratory, #004623)^{86 (link)} and Axin2^LacZ/+ (The Jackson Laboratory, #009120)^{87 (link)} mice were obtained from The Jackson Laboratory and crossed with Dhcr7^−/− mouse line to generate Dhcr7^−/−;Topgal and Dhcr7^+/+;Topgal, Dhcr7^−/−;Axin2^LacZ/+, and Dhcr7^+/+;Axin2^LacZ/+ mice. Genotyping was performed using PCR primers, as previously described.^{12 (link),22 (link),85 (link)} Pregnant females were treated with simvastatin (S6196; Sigma-Aldrich) at a dose of 10 mg/kg⁻¹ body weight (BW) from E12.5 to E18.5, or from day 7 to day 42, administered by intraperitoneal injection.

Suzuki A., Ogata K., Yoshioka H., Shim J., Wassif C.A., Porter F.D, & Iwata J. (2020). Disruption of Dhcr7 and Insig1/2 in cholesterol metabolism causes defects in bone formation and homeostasis through primary cilium formation. Bone Research, 8, 1.

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Genetically Modified Mouse Models

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Stanford APLAC approved all procedures (#13146), which conform to ARRIVE guidelines. C57BL/6 and Axin2^LacZ/+ mice were obtained from Jackson Laboratory (Bar Harbor, ME). Axin2^CreERT2/+; R26^mTmG/+ mice were generated from Axin2^CreERT2/+ and R26^mTmG/+ mice, both from Jackson Labs (#18867 and #7676). Nude mice (#086) were purchased from Charles River and Beta-actin-enhanced green fluorescent protein (ACTB-eGFP) transgenic mice (#027) were purchased from the Jackson Laboratory (Sacramento, California).

Chen T., Li J., Córdova L.A., Liu B., Mouraret S., Sun Q., Salmon B, & Helms J. (2018). A WNT protein therapeutic improves the bone-forming capacity of autografts from aged animals. Scientific Reports, 8, 119.

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Genetic Mouse Strains for Developmental Studies

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The following genetic mouse strains were obtained from The Jackson Laboratory, housed at Cincinnati Children’s Hospital Research Foundation animal facility, and maintained according to IACUC protocol (0B09074): Axin2:LacZ (stock no. 009120), Shh:Cre (stock no. 005622), and β-catenin^floxed (stock no. 004152). Timed matings, with the morning the vaginal plug was observed being denoted as E0.5, were used to generate embryos at various stages that were harvested for either wholemount staining or tissue dissection. At least two litters of embryos were analyzed at each developmental stage examined. Both male and female embryos were analyzed.

McCracken K.W., Zhang X, & Wells J.M. (2017). Wnt/β-catenin promotes gastric fundus specification in mice and humans. Nature, 541(7636), 182-187.

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Generation and Characterization of Transgenic Mouse Lines

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The generation of and genotyping information regarding the R26R^EYFP, R26R^tdTomato, Pdgfra^EGFP, Pdgfra^CreERT2 and Axin2^LacZ mouse lines have been previously described and were all purchased from Jackson Laboratories. The Axin2^CreERT2-tdT mouse line was generated as described (Frank et al., 2016 (link)). The Wnt2^CreERT2 mouse was described previously (Peng et al., 2013 (link)). The Sftpc^CreERT2 mouse line was generously provided by Dr. Hal Chapman (Chapman et al., 2011 (link)). The Ctnnb1^lox(ex3) allele was described previously (Harada et al., 1999 (link)). Unless otherwise indicated all mice were maintained on a mixed background and were 6–8 weeks of age for all experiments described in this study. All animal procedures were performed under the guidance of the University of Pennsylvania Institutional Animal Care and Use Committee.

Zepp J.A., Zacharias W.J., Frank D.B., Cavanaugh C.A., Zhou S., Morley M.P, & Morrisey E.E. (2017). Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung. Cell, 170(6), 1134-1148.e10.

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Genetic Mouse Models for Research

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Mice were derived and maintained at the Stanford University Research Animal Facility in accordance with Stanford University guidelines. All the animals were housed in sterile micro-insulators and given water and rodent chow ad libitum. Actin^CreER transgenic mice were a gift from Dr. Liqun Luo (Stanford University). Axin2^lacZ and Axin2^CreER (The Jackson Laboratory, strain name: B6.129(Cg)-Axin2^{tm1(cre/ERT2)Rnu}/J, stock number 018867) were a gift from Dr. Roel Nusse (Stanford University). R26^mT/mG transgenic mice were obtained from The Jackson Laboratory, strain name: B6.129(Cg)-Gt(ROSA)26Sor

Rinkevich Y., Montoro D.T., Contreras-Trujillo H., Harari-Steinberg O., Newman A.M., Tsai J.M., Lim X., Van-Amerongen R., Bowman A., Januszyk M., Pleniceanu O., Nusse R., Longaker M.T., Weissman I.L, & Dekel B. (2014). In vivo Clonal Analysis Reveals Lineage-Restricted Progenitor Characteristics in Mammalian Kidney Development, Maintenance and Regeneration. Cell reports, 7(4), 1270-1283.

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Conditional Gfi1b Deletion in Megakaryocytes

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Gfi1b^fl/fl mice^{9 (link)} were crossed with Rosa-CreERT2, and Axin2^+/LacZ (Jackson, B6.129P2-Axin2tm1Wbm/J) or TCF/Lef:H2B-GFP (Jackson, 61Hadj/J) transgene reporter line. PF4-cre mice were used to delete Gfi1b in MKs for immunofluorescence microscopy analysis. Age and sex-matched mice were used in all experiments. For BM transplantation C57B6 CD45.1 mice were used. All mice were housed under SPF conditions and the IRCM Institutional Review Board approved all animal protocols and experimental procedures were performed in compliance with IRCM and CCAC (Canadian Council of Animal Care) guidelines.

Shooshtarizadeh P., Helness A., Vadnais C., Brouwer N., Beauchemin H., Chen R., Bagci H., Staal F.J., Coté J.F, & Möröy T. (2019). Gfi1b regulates the level of Wnt/β-catenin signaling in hematopoietic stem cells and megakaryocytes. Nature Communications, 10, 1270.

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Genetic Lineage Tracing in Mice

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PDGFRαCreER mice (Collins et al., 2011) were bred to β-cat^fl/fl (Brault et al., 2001) and β-cat^fl(Ex3/+)) (Harada et al., 1999 (link)) mice to generate PDGFRαCreER;β-cat^fl/fl and PDGFRαCreER;β-cat^fl(Ex3/+) mice respectively. Axin2CreER (van Amerongen et al., 2012 (link)) mice were bred to Gt(ROSA)26Sortm4(ACTB-tdTomato–eGFP)Luo/J (mTmG) (Muzumdar et al., 2007 (link)). Reporter lines used to visualize Cre-recombination Gt(ROSA)26Sortm4(ACTB-tdTomato–eGFP)Luo/J (mTmG), Axin2LacZ (Lustig et al., 2002), PDGFRαH2BGFP (Hamilton et al., 2003 (link)) Sox2eGFP (Arnold et al., 2011 (link)) mice were obtained from Jackson Laboratory. R26Fucci2aR reporter mice were used to visualize the cell cycle phases (Mort et al., 2014 (link)). A random population of both male and female mice were used for all experiments, as it was not possible to sex the animals due to developmental age. All procedures involving animal subjects were performed under the approval of the Institutional Animal Care and Use Committee of the Yale School of Medicine.

Gupta K., Levinsohn J., Linderman G., Chen D., Sun T.Y., Dong D., Taketo M.M., Bosenberg M., Kluger Y., Choate K, & Myung P. (2018). Single-cell analysis reveals a hair follicle dermal niche molecular differentiation trajectory that begins prior to morphogenesis. Developmental cell, 48(1), 17-31.e6.

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Genetic Mouse Strains for Developmental Studies

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McCracken K.W., Zhang X, & Wells J.M. (2017). Wnt/β-catenin promotes gastric fundus specification in mice and humans. Nature, 541(7636), 182-187.

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Mouse Lineage Tracing in Intestinal Injury

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All mice were maintained in compliance with Institutional Animal Care and Use Committee (IACUC) guidelines of MD Anderson Cancer Center. Male and female mice (older than six weeks) were used for experiments. Gt(ROSA) 26Sor^tm1(EYFP)Cos/J (Jax strain 006148), Gt(ROSA)26Sor^tm1(DTA)Lky/J (Jax strain 009669), Ctnnb1^tm2Kem/KnwJ (Jax strain 004152), and Axin2^LacZ (Jax strain 009120) were purchased from Jackson Laboratory. Genotyping was performed following the Jackson Laboratory’s protocol. Tam was dissolved in corn oil (Fisher) at a final concentration of 10 mg/ml for intraperitoneal (i.p.) administration (50 mg/kg). For intestine injury, mice were exposed to 10 Gy or 6 Gy (non-lethal dose for cell labeling) WBI, and tissues were collected at multiple time points.

Suh H.N., Kim M.J., Jung Y.S., Lien E.M., Jun S, & Park J.I. (2017). Quiescence Exit of Tert+ Stem Cells by Wnt/β-Catenin Is Indispensable for Intestinal Regeneration. Cell reports, 21(9), 2571-2584.

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Unilateral Ureteral Obstruction Model

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Male C57BL/6 or Axin2-LacZ (Jackson labs strain, B6.129P2-Axin2tm1Wbm/J) mice weighing approximately 20 to 22 g were used for the studies. For unilateral ureteral obstruction (UUO), after inhaled anesthesia, a mid-line incision was made, through which the left kidney was isolated. The ureter was isolated 3–5 mm below the kidney and tied off using 5-0 silk. The skin and muscle were then closed with continuous 4-0 silk sutures. The first dose of C59 (25 mg/kg) was given immediately before the surgery and then repeated once daily on days 1–6 after surgery. On day 3 or 7 following UUO the mice were euthanized and the obstructed and contralateral non-obstructed kidneys were harvested for analysis.

Madan B., Patel M., Zhang J., Bunte R., Rudemiller N.P., Griffiths R., Virshup D.M, & Crowley S.D. (2016). Experimental inhibition of porcupine mediated Wnt O-acylation attenuates kidney fibrosis. Kidney international, 89(5), 1062-1074.

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