Anti cnot1 14276 1 ap

The following antibodies were used for IP and/or western blot analysis: anti-β-actin (#4970; Cell Signalling), anti-CDK9 (sc-13130; Santa Cruz), anti-CNOT1 (14276-1-AP; Protein Tech), anti-CNOT7 (H00029883; Abnova), anti-FLAG (M2; Sigma), anti-hnRNPD (Q14103; Millipore), anti-HA (1867423; Roche), anti-IGF2BP1 (sc-21026; Santa Cruz), anti-KHSRP (ab83291; Abcam), anti-LARP7 (LaRP7-101AP; FabGennix Inc.), anti-LDLR (AF2148; BD Biosciences), anti-Myc (9E10; Roche), anti-phospho-ERK (#9101s; Cell Signalling), anti-phospho-MAPKAPK2 (#3007s; Cell Signalling), anti-phospho-RSK (sc-17033; Santa Cruz), anti-ZFP36L1 (#2119; Cell Signalling), anti-phospho-S6 (#2211; Cell Signalling).

Whole-cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (ThermoFisher Scientific). Immunoblotting was performed with anti-MUC1-C (#16564S, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-NF-κB p65 (#8242S, 1:1000 dilution; CST), anti-phospho-NF-κB p65 (#3037s, 1:1000 dilution; CST), anti-GAPDH (5174, 1:1000 dilution; CST), anti-RBM15B (22249-1-AP, 1:1000 dilution; Proteintech, Rosemont, IL, USA), anti-RBM15 (10587-1-AP, 1:5000 dilution; Proteintech), anti-YTHDF2 (24744-1-AP, 1:5000 dilution; Proteintech), anti-CNOT1 (14276-1-AP, 1:1000 dilution; Proteintech), anti-WTAP (#56501, 1:1000 dilution; CST), anti-METTL3 (#96391, 1:1000 dilution; CST), anti-METTL14 (#51104, 1:1000 dilution; CST), anti-IGF2BP1 (22803-1-AP, 1:5000 dilution; Proteintech), anti-TDP-43 (#32654, 1:1000 dilution; CST), anti-NOTCH1 (#3608S, 1:1000 dilution; CST), anti-BMI1 (#6964P, 1:1000 dilution; CST), anti-CD44 (#5640S, 1:1000 dilution; CST), anti-β-actin (A5441; 1:50000 dilution; Sigma, St. Louis, MO, USA) and anti-tubulin (#2144S, 1:1000 dilution; CST). Signals shown in immunoblots and in a separate biologic replicate were each scanned in triplicate. The results (mean ± SD of six determinations) are expressed as relative signal intensity compared to that obtained for GAPDH.