Ir806

IR806, BSA, PDI (P3818), DTT (D0632), PDI inhibitor 16F16 (SML0021) were purchased from Sigma-Aldrich. Pentapeptide CREKA were customized from GL biochem (shanghai) Ltd. Rt-PA (Actilyse®/Alteplase) was obtained from Zhongda Hospital of Wuhan University. The recombinant Anti-Fibrinogen alpha chain (ab92572; 1:400 diluted) was purchased from Abcam, and Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H ​+ ​L) (711-165-152; 1:400 diluted) was purchased from Jackson ImmunoResearch. All other standard reagents were purchased from commercial suppliers (such as Ponsure Biological) and used without further purification. NIH3T3 (mouse fibroblast cell line) cells, and RAW264.7 (mouse macrophages cell line) cells were obtained from Key Gene Tech Co. (Shanghai, China), cultured in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, U.S.), supplemented with 10% FBS (Gibco, Grand Island, NY, USA), at 37 ​°C in a humidified incubator containing 5% CO₂.

Heptamethine cyanine dyes (IR-775, IR-780, IR-783, IR-797, IR-806, IR-808), Intralipid and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis., MO, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). Calcein-AM and propidium iodide (PI), fetal bovine serum (FBS), DMEM, trypsin-EDTA and penicillin-streptomycin were purchased from Gibco Life Technologies (AG, Switzerland). All other chemicals used in this study were of analytical reagent grade and used without further purification. Milli-Q grade (R > 18 MΩ cm) water was used throughout. Human glioblastoma luciferase-labeled U87 cell lines (U87L) were purchased from American Type Culture Collection (ATCC, Manassas, VA).