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Dmi8 microscope

Manufactured by Zeiss
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The DMi8 microscope is a high-performance, inverted microscope system designed for advanced imaging and analysis applications. It features a modular design, allowing for customization to meet the specific needs of researchers and laboratories. The DMi8 provides stable and precise optical performance, enabling users to capture detailed images and conduct accurate measurements.

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Lab products found in correlation

Lsm 700 confocal laser scanning microscope, by Zeiss (3 mentions) Alexa fluor 594 affinipure goat anti rabbit igg h l, by Jackson ImmunoResearch (2 mentions) Alexa fluor 488 affinipure goat anti mouse igg h l, by Jackson ImmunoResearch (2 mentions) Ag 25b 0006, by Adipogen (1 mentions) Ventana discovery ultra, by Roche (1 mentions) Omnimap anti mouse hrp, by Roche (1 mentions) Confocal microscope, by Zeiss (1 mentions) Postn, by Abcam (1 mentions) Mmp 2, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions) Donkey anti goat igg, by Thermo Fisher Scientific (1 mentions)

5 protocols using dmi8 microscope

1

SARS-CoV-2 Infection and Lung Pathology

Cited 1 time
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Tissues were obtained from autopsy of 11 patients with PCR confirmed SARS-CoV-2 and death due to COVID-related pneumonia and diffuse alveolar damage. Lung tissue from 3 patients with death due to non-pulmonary-related causes and no evidence of pneumonia or other pulmonary histopathological abnormalities were used as control. Tissues were fixed in 10% buffered formalin and paraffin embedded (FFPE). Immunofluorescence staining was performed on the Ventana Discovery Ultra instrument (Roche). Tissue sections were deparaffinized and antigen retrieval was performed using citrate buffer (pH = 8.0) at 72 oC (CC1; Roche). Tissue sections were first probed with rabbit anti-ASC (AG-25B-0006, AdipoGen) followed by anti-Rabbit HRP (760-4311, Roche) and fluorescent visualization using tyramide signal amplification (Red610, Roche). Residual peroxidase was blocked (760-4840, Roche), followed by application of mouse anti-CD68 (KP-1, Roche), OmniMap anti-mouse HRP (Roche) and visualization using Discovery Rhod6G (Roche). Representative immunofluorescence images were acquired using a Leica DMi8 microscope and for analysis the slides were scanned using a Zeiss AxiosScan Z1. Staining and imaging were performed by the Cedars-Sinai Biobank and Translational Research Core. ASC speck quantification was performed using open source QuPath software (version 0.2.3) (Bankhead et al., 2017 (link)).
Xian H., Liu Y., Rundberg Nilsson A., Gatchalian R., Crother T.R., Tourtellotte W.G., Zhang Y., Aleman-Muench G.R., Lewis G., Chen W., Kang S., Luevanos M., Trudler D., Lipton S.A., Soroosh P., Teijaro J., de la Torre J.C., Arditi M., Karin M, & Sanchez-Lopez E. (2021). Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation. Immunity, 54(7), 1463-1477.e11.
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2

Immunofluorescence Assay for GR and NR2F1

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LNCaP/AR cells were seeded on round glass coverslips. After 24 hr, cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min at room temperature, followed by permeabilization with 0.5% Triton X-100 for 5 min. Then cells were incubated with primary antibodies (Rabbit anti-GR, CST, #12041; mouse anti-NR2F1 R&D, PP-H8132-00), overnight at 4°C after blocking with 3% BSA/PBS for 30 min at room temperature, followed by incubation with Alexa Fluor-labeled secondary antibodies (Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L), Jackson Immunoresearch; Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L), Jackson Immunoresearch) for 1hr at room temperature. Nuclei were stained with DAPI. Images were acquired on Leica DMi8 microscope and Zeiss LSM 700 confocal Laser Scanning Microscope. Three biological replicated, representative images of each cell line were used to quantify the fluorescence intensity of GR and NR2F1 signals, using imageJ.
Zhang Z., Zhou C., Li X., Barnes S.D., Deng S., Hoover E., Chen C.C., Lee Y.S., Zhang Y., Wang C., Metang L.A., Wu C., Tirado C.R., Johnson N.A., Wongvipat J., Navrazhina K., Cao Z., Choi D., Huang C.H., Linton E., Chen X., Liang Y., Mason C.E., de Stanchina E., Abida W., Lujambio A., Li S., Lowe S.W., Mendell J.T., Malladi V.S., Sawyers C.L, & Mu P. (2020). Loss of CHD1 Promotes Heterogeneous Mechanisms of Resistance to AR-Targeted Therapy via Chromatin Dysregulation. Cancer Cell, 37(4), 584-598.e11.
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3

Immunofluorescence Analysis of GR and NR2F1

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LNCaP/AR cells were seeded on round glass coverslips. After 24 hr, cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min at room temperature, followed by permeabilization with 0.5% Triton X-100 for 5 min. Then cells were incubated with primary antibodies (Rabbit anti-GR, CST, #12041; mouse anti-NR2F1 R&D, PP-H8132–00), overnight at 4°C after blocking with 3% BSA/PBS for 30 min at room temperature, followed by incubation with Alexa Fluor-labeled secondary antibodies (Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L), Jackson Immunoresearch; Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L), Jackson Immunoresearch) for 1hr at room temperature. Nuclei were stained with DAPI. Images were acquired on Leica DMi8 microscope and Zeiss LSM 700 confocal Laser Scanning Microscope. Three biological replicated, representative images of each cell line were used to quantify the fluorescence intensity of GR and NR2F1 signals, using imageJ.
Zhang Z., Zhou C., Li X., Barnes S.D., Deng S., Hoover E., Chen C.C., Lee Y.S., Zhang Y., Wang C., Metang L.A., Wu C., Tirado C.R., Johnson N.A., Wongvipat J., Navrazhina K., Cao Z., Choi D., Huang C.H., Linton E., Chen X., Liang Y., Mason C.E., de Stanchina E., Abida W., Lujambio A., Li S., Lowe S.W., Mendell J.T., Malladi V.S., Sawyers C.L, & Mu P. (2020). Loss of CHD1 Promotes Heterogeneous Mechanisms of Resistance to AR-Targeted Therapy via Chromatin Dysregulation. Cancer Cell, 37(4), 584-598.e11.
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4

Protein Localization and Quantification

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PAECs were fixed with NBF for 5 min and washed with PBS. For permeabilization, cells were incubated with 0.1% Triton X-100 in PBS for 5 min. After blocking with 1% BSA in PBS for 1 h, the primary antibodies were applied overnight at 4 °C. FN (1:200; BD Biosciences, Franklin Lakes, NJ, USA), MMP2 (1:200; Cell Signaling Technology), and POSTN (1:300; Abcam) antibodies were used. The secondary antibodies used were goat anti-rabbit Alexa Fluor 488 and 568. Images were obtained using a Leica DMI8 microscope and a Zeiss confocal microscope.
Lee D., Lee H., Jo H.N., Yun E., Kwon B.S., Kim J, & Lee A. (2024). Endothelial periostin regulates vascular remodeling by promoting endothelial dysfunction in pulmonary arterial hypertension. Animal Cells and Systems, 28(1), 1-14.
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5

Immunofluorescence analysis of DNA repair proteins

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5000 LNCaP/AR cells were seeded on round glass coverslips. After 24h, cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min at room temperature, followed by permeabilization with 0.5% Triton X-100 for 5 min. Then cells were incubated with primary antibodies overnight at 4°C after blocking with 3% BSA/PBS for 30 min at room temperature, followed by incubation with Alexa Fluor-labeled secondary antibodies for 1 h at room temperature. Nuclei were stained with DAPI. Images were acquired on Leica DMi8 microscope and Zeiss LSM 700 confocal Laser Scanning Microscope. Antibody for IF: Rabbit anti-SYNCRIP, Sigma, HPA041275; Rabbit anti-γH2AX Ser139, CST #2577; Rabbit anti-APOBEC3B, abcam, ab191695, Rabbit anti 53BP1, CST #4937, Rabbit anti-APOBEC1 (Millipore, ABE438), Goat anti APOBEC3A (Sigma-aldrich, #SAB2501944), Rabbit anti-APOBEC3B mAb (CST, #41494), Rabbit anti-APOBEC3C (Proteintech, 10591-1-AP), Mouse anti flag M2 (Sigma, F3165). Goat Anti-Rabbit IgG Antibody (Alexa Fluor 488, Jackson ImmunoResearch, 111-545-003), Goat Anti-Rabbit IgG Antibody (Alexa Fluor 594, Jackson ImmunoResearch, 111-585-003), Donkey anti-Goat IgG (Alexa Fluor 488, Thermo Scientific, A-11055).
Shen C., Buck A., Mehrke G., Polat B., Gross H.J., Bachem M, & Reske S. (2001). Triplex forming oligonucleotide targeted to 3′UTR downregulates the expression of the bcl-2 proto-oncogene in HeLa cells. Nucleic Acids Research, 29(3), 622-628.
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