P57kip2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

P57Kip2 is a cell cycle inhibitor protein that regulates the transition from G1 to S phase in the cell cycle. It acts by binding to and inhibiting the activity of cyclin-dependent kinase (CDK) complexes, thereby preventing cell cycle progression.

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2 protocols using p57kip2

Antibody Sources for Western Blot

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Primary antibodies were obtained from the following sources: Na⁺/K⁺ ATPase‐α, calnexin, calpain 2, CDK2, cyclin B1, cyclin E, ERK2, lamin B, HA tag, p21, p27^Kip1, p57^Kip2, β‐tubulin from Santa Cruz Biotechnology (Santa Cruz, CA), β‐actin from Chemicon International (Billerica, MA), phospho‐AKT (Ser473), AKT, phosphorylated ERK1/2 (T202/Y204), and Lamp1 from Cell Signaling Technology (Danvers, MA) and Cathepsin B from R&D Systems (Mississauga, ON, Canada). Mouse and rabbit horseradish peroxidase antibodies were purchased from Amersham Biosciences (Pittsburg, PA), goat horseradish peroxidase antibody from Santa Cruz, and alkaline phosphatase‐conjugated antibodies were purchased from Promega (Madison, WI).

Bian B., Mongrain S., Cagnol S., Langlois M., Boulanger J., Bernatchez G., Carrier J.C., Boudreau F, & Rivard N. (2015). Cathepsin B promotes colorectal tumorigenesis, cell invasion, and metastasis. Molecular Carcinogenesis, 55(5), 671-687.

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Isolation and Characterization of Pancreatic Cells

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Barium chloride and collagenase type 2 solution (210 U/mL), were purchased from Sigma-Aldrich, St. Louis, USA. Pancreatin solution (0.6 mg/mL) was purchased from Thermo Fisher Scientific, Waltham, USA. An antibody for Akt (catalogue number #9272) was purchased from Cell Signaling Technology, Inc., Danvers, USA. Antibodies for Ser 473 phosphorylated Akt1 (catalogue number sc-514032), an antibody BrdU (catalogue number sc-32323) p27^Kip1 (catalogue number sc-1641) and p57^Kip2 (catalogue number sc-56341) were purchased from Santa Cruz Biotechnology, Dallas, USA. BIO ((2′Z,3′E)-6-Bromoindirubin-3′-oxime) was kindly provided by Professor Yong-Chul Kim, Laboratory of Drug Discovery, Gwangju Institute of Science and Technology, Korea.

Kim A.R., Kim S.W., Lee B.W., Kim K.H., Kim W.H., Seok H., Lee J.H., Um J., Yim S.H., Ahn Y., Jin S.W., Jung D.W., Oh W.K, & Williams D.R. (2020). Screening ginseng saponins in progenitor cells identifies 20(R)-ginsenoside Rh2 as an enhancer of skeletal and cardiac muscle regeneration. Scientific Reports, 10, 4967.

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