NK cells were depleted by intraperitoneal administration of anti‐asialo GM1 pAb (cat#146002; BioLegend). Normal rabbit serum (cat#140–06571; Wako) was used as a control. In brief, antibodies (50 μL) were administered intraperitoneally 1 day before transplantation of B16 melanoma cells.
Normal rabbit serum
Manufactured by Fujifilm
Sourced in Japan
Normal rabbit serum is a laboratory reagent used as a control in various immunological and biochemical assays. It is derived from the blood of healthy rabbits and contains a natural mixture of proteins, antibodies, and other components found in rabbit serum.
Automatically generated - may contain errors
Lab products found in correlation
Control mouse igg2a c1.18.4, by BioXCell (2 mentions)
Anti asialo gm1 antiserum, by Fujifilm (1 mentions)
Anti cd4 mab clone gk1, by BioXCell (1 mentions)
Anti cd8 mab clone 2, by BioXCell (1 mentions)
Anti cd3 mab clone 17a2, by BioLegend (1 mentions)
Anti cd8 mab clone 53 6.7, by BioLegend (1 mentions)
4 protocols using normal rabbit serum
1
Depleting NK Cells for Melanoma Study
2
Selective Depletion of NK Cells
3
In Vivo Cell Depletion Protocol
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For the depletion of CD4+ T cells and CD8+ T cells in vivo, 200 µg of anti-CD8 mAb (clone 2.43; BioXcell) and anti-CD4 mAb (clone GK1.5; BioXcell) were injected intraperitoneally into mice four times at days 1, 3, 5, and 7 before challenge. Non-depleted control mice were given isotype control rat IgG2b antibodies (clone LTF-2, BioXcell). Blood and lungs were taken from the mice 24 h after the last antibody injection and were subjected to flow cytometry to confirm the depletion. For flow cytometric analysis, we used anti-CD8 mAb (clone 53-6.7; Biolegend) and anti-CD4 mAb (clone RM4-5; Biolegend) directed against different epitope of CD4 and CD8 molecules to that of the depleting antibodies. For the depletion of NK cells, 20 µl of anti-asialo GM1 antiserum (Wako Pure Chemical Industries) were injected intraperitoneally into mice four times at days 1, 3, 5, and 7 before challenge. Non-depleted control mice were given normal rabbit serum (Wako Pure Chemical Industries). The spleens were taken from the mice 24 h after the last antibody injection to confirm the depletion by flow cytometry. For flow cytometric analysis, we used anti-CD3 mAb (clone 17A2; Biolegend) and anti-CD49b mAb (clone DX5; Biolegend).
4
Selective Depletion of NK Cells
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One day before infection, selective depletion16 (link) of NK cells was attained through a single intraperitoneal (i.p.) injection of 25 μg/mouse anti-NK1.1 monoclonal antibody (PK136) or 25 μg/mouse of a control mouse IgG2a (C1.18.4) produced by Bio-X-Cell (West Lebanon, NH). Alternatively, NK Cells were depleted through a single i.p. injection with 20 μL of anti-asialo GM1 (Wako Biochemicals, Richmond, VA) diluted in 180 μL of Hank’s balanced salt solution (HBSS) or 20 μL of normal rabbit serum (Wako Pure Chemical Industries, Japan) diluted in 180 μL HBSS.
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