Anti cleaved caspase 3 clone 5a1e

Poly(I:C) (high molecular weight) was from Invivogen (San diego, CA, USA) and was used at 100 µg/ml unless otherwise stated. Z-VAD-FMK (R&D systems, Minneapolis, MN, USA) was used at 20 µM. Smac mimetic BV6 was first a gift from Ira Mellman and Wayne Fairbrother (Genentech, CA, USA), and later purchased from Selleckchem (Houston, TX, USA), and was used at 5 µM. Paclitaxel and necrostatin-1 were purchased from Sigma-Aldrich. RIPK3 kinase inhibitor GSK'872 was from Merck-Calbiochem (Darmstadt, Germany).
Antibodies were purchased from the following companies: anti-caspase-8 (clone C-20) (Santa CruzBiotechnology, Dallas, TX, USA), anti-actin (clone C4) (MP Biomedicals Europe, Illkirch, France), anticIAP1/2 (clone 315301) (R&D systems), anti-RIPK1 (clone 38) (BD Biosciences, San Jose, CA, USA), anti-FLIP (clone NF6) (Alexis Biochemicals, San Diego, CA, USA) anti-TRIF (Cell Signaling Technology, Danvers, MA, USA), anti-Bcl-xL (Cell Signaling), anti-TLR3 (cloneD10F10) (Cell Signaling) and clone 1210F1 (Dendritics, Lyon, France), anti-caspase-8 (clone 1C12) (Cell Signaling), anti-caspase-3 (Cell Signaling), anti-cleaved caspase 3 (clone 5A1E) (Cell Signaling), and anti-RIPK3 (clone E1Z1D) (Cell Signaling).