Biotinylated NPDP15 and NANP18 peptides were diluted (20 nM) in HEPES buffered saline (HBS) (10 mM HEPES, pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant Tween-20). HBS was also used as running buffer. An irrelevant biotinylated 19-mer peptide was used as a control for non-specific interactions. A NeutrAvidin-immobilized NLC ProteOn sensor chip (Biorad) was pre-conditioned with an NaCl solution (1 M) and the biotinylated peptides were injected onto the chip. The monoclonal antibodies were diluted and titrated in HBS (50-16.7-5.6-1.9-0.6 nM) and injected onto chip; one channel of the chip was injected with HBS and used as reference for the analysis. All injections were made at a flow rate of 100 μl/min. Injection time and dissociation time were 240 s and 900 s, respectively. Each binding interaction of the monoclonal antibodies with the biotinylated peptides was assessed using a ProteON XPR36 instrument (Biorad) and data were processed with ProteOn Manager Software (version 3.1.0.6). Ka, Kd and KD were calculated by applying the Langmuir fit model.
Neutravidin immobilized nlc proteon sensor chip
Manufactured by Bio-Rad
The NeutrAvidin-immobilized NLC ProteOn sensor chip is a lab equipment product designed for use in the ProteOn XPR36 interaction array system. The sensor chip features a NeutrAvidin surface, which is used to capture biotinylated molecules for subsequent analysis of molecular interactions.
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Lab products found in correlation
2 protocols using neutravidin immobilized nlc proteon sensor chip
1
Kinetic Analysis of Monoclonal Antibodies
2
Kinetic Analysis of Monoclonal Antibodies
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Biotinylated NPDP15 and NANP18 peptides were diluted (20 nM) in HEPES buffered saline (HBS) (10 mM HEPES, pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant Tween-20). HBS was also used as running buffer. An irrelevant biotinylated 19-mer peptide was used as a control for non-specific interactions. A NeutrAvidin-immobilized NLC ProteOn sensor chip (Biorad) was pre-conditioned with an NaCl solution (1 M) and the biotinylated peptides were injected onto the chip. The monoclonal antibodies were diluted and titrated in HBS (50-16.7-5.6-1.9-0.6 nM) and injected onto chip; one channel of the chip was injected with HBS and used as reference for the analysis. All injections were made at a flow rate of 100 μl/min. Injection time and dissociation time were 240 s and 900 s, respectively. Each binding interaction of the monoclonal antibodies with the biotinylated peptides was assessed using a ProteON XPR36 instrument (Biorad) and data were processed with ProteOn Manager Software (version 3.1.0.6). Ka, Kd and KD were calculated by applying the Langmuir fit model.
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