Actinomycin D (ActoD) (Sigma-Aldrich) was used at a concentration of 5 µg/mL to induce apoptosis. The pan-caspase inhibitor z.vad.FMK (BD Pharmigen) was used at concentration of 20 µM. Caspase inhibitors were purchased from Calbiochem and used at 4 µM (caspase 8 inhibitor) or as indicated. DMSO only controls were used to account for any cellular or viral alterations induced by the drug vehicle. The HRV 3C protease inhibitor, Rupintrivir (Santa Cruz, SC-208317) was added to infected cells at 1 µM as indicated. Anti-RIPK1 (ThermoScientific, 1860909) or Anti-RIPK1 n-terminal (Cell signalling 3493) antibodies were used to detect full length RIPK1 and its cleavage products. Antibodies to caspase 3 (9662), Poly(A)-binding protein (PABP, 4992) and tubulin (2148) were purchased from Cell Signalling Technologies. Antibodies to poly-histidine tag (11922416001) Green Fluorescent protein (GFP, 11814460001) were purchased from Roche. Viral protein expression was detected with anti-VP2 antibody (QED Biosciences, 18758). Rabbit polyclonal antibody to eIF4G (sc11373) was purchased from Santa Cruz.
Anti ripk1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-RIPK1 is a laboratory reagent used in research applications. It functions as an inhibitor of the RIPK1 protein, which plays a role in cellular processes. This product is intended for research use only and its specific applications should be determined by the end user.
Automatically generated - may contain errors
Lab products found in correlation
Rupintrivir, by Santa Cruz Biotechnology (1 mentions)
Anti inos, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Elabscience (1 mentions)
Anti caspase 8, by Thermo Fisher Scientific (1 mentions)
Anti tlr4, by Santa Cruz Biotechnology (1 mentions)
Anti gal 3 antibody, by Santa Cruz Biotechnology (1 mentions)
Fluorescent microscope, by Olympus (1 mentions)
3 protocols using anti ripk1
1
Apoptosis Induction and Signaling Inhibition
2
Carvedilol Mitigates Inflammatory Responses
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Carvedilol was purchased from GNP company (6th of October, Giza, Egypt). Cadmium chloride (CdCl2) and ACET were purchased from Sigma Aldrich (St. Louis, MO, USA). Rat tumor necrosis factor-alpha (TNF-α; CAT# E-EL-R2856), troponin-I (CAT# E-CL-R0721), and (IL-6; CAT# E-EL-R0015) ELISA kits were purchased from ELABSCIENCE (Wuhan, China). Primers for Sirtuin 1 (SIRT1), Forkhead box O3 (FOXO3), KEAP-1, Nrf2, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes were synthesized by Vivantis Technologies (Malaysia). Anti-RIPK1 (CAT# PA5-20811), anti-RIPK3 (CAT# PA5-19956), anti-MLKL (CAT# PA5-115578), and anti-caspase-8 (CAT# PA5-20118) were purchased from ThermoFisher Scientific (USA). Anti-iNOS (CAT# sc-7271), IκB (CAT# sc-1643), and anti-TLR4 (CAT# sc-293072) were purchased from Santa Cruz (USA). Anti-NF-κB (CAT# abx012874) was purchased from Abbexa (Germany). Anti-NADPH oxidase (CAT# E-AB-70215) and anti-β-actin (CAT# E-AB-20031) were purchased from ELABSCIENCE (China). Anti-Nrf2 (CAT# YPA1865) and anti-HO-1 (CAT# YPA1919) were purchased from Biospes (China). ACET and CV dissolved in 0.5% carboxymethyl cellulose (CMC).
3
Immunofluorescence Staining of Apoptosis Markers
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Five-μm sections were deparaffinized with xylene and rehydrated with graded alcohol. Sections were placed in Retrieval Solution with a high pH (pH 9) in a water bath at 95°C for 20 minutes. Sections were then washed with distilled water for 5 minutes followed by TBST buffer for 5 minutes. Sections were then incubated with anti-GAL-3 antibody (Rabbit Polyclonal,1:50, Santa Cruz Biotechnology, Dallas, Texas, USA), anti-RIPK1 (Rabbit polyclonal,1:50, Thermo Scientific, Thermo Scientific, Waltham, MA, USA), anti-RIPK3 1 (Rabbit polyclonal,1:50, Thermo Scientific, Thermo Scientific, Waltham, MA, USA), and anti-MLKL 1 (Rabbit monoclonal, 1:50, Thermo Scientific, Thermo Scientific, Waltham, MA, USA) overnight at room temperature. Sections were subsequently incubated with donkey anti-rabbit Alexa Fluor 488, (Jackson Immune Research Laboratories, Bar Harbor, Maine, USA, 1:100) antibody. Finally, sections were mounted in DAPI-watersoluble mounting media (Abcam, Cambridge, MA, USA) and viewed with Olympus Fluorescent microscope. Appropriate positive control sections were used. For negative control, the primary antibody was not added to sections and the whole procedure carried out in the same manner as mentioned above. Positive and negative controls were used in every batch of stained slides (not shown in figures).
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