Protein extracts were obtained by lysing cells. Samples were fractionated by SDS-PAGE and transferred to polyvinylidene-fluoride (PVDC) membrane. After blocking with 5 % milk in PBST (phosphate-buffered saline with 0.1 % tween 20) for 1 h at room temperature, the membranes were probed with the corresponding primary and secondary antibodies. And the following antibodies were used: anti-HLA DR+DP+DQ (1:200, Abcam), anti-CIITA (1:200, Santa Cruz Biotechnology) and anti-β-Actin (1:5000, HuanAn).
Anti hla dr dp dq
Manufactured by Abcam
Anti-HLA DR+DP+DQ is a laboratory product used to detect the expression of human leukocyte antigen (HLA) class II molecules, including HLA-DR, HLA-DP, and HLA-DQ, on the surface of cells. This product is intended for research use only and its core function is to facilitate the identification and analysis of cells expressing these HLA class II molecules.
Automatically generated - may contain errors
2 protocols using anti hla dr dp dq
1
Western Blot Analysis of HLA and CIITA
2
Immunochemistry Profiling of Pluripotent Markers
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Immunochemistry was performed as previously described [29 (link)]. The primary antibodies used were anti-Oct4 (1:100, Santa Cruz Biotechnology), anti-Nanog (1:150, Santa Cruz Biotechnology), anti-Tra-1-60 (1:150, Chemicon), anti-SSEA3 (Ascites, 1:400, Developmental Studies Hybridoma Bank), anti-CIITA (1:200, Santa Cruz Biotechnology) and anti-HLA DR+DP+DQ (1:200, Abcam) for staining.
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