HET0016, DDMS and 20-HETE were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Human recombinant macrophage colony-stimulating factor (M-CSF), mouse recombinant IL-4 and IL-13 were purchased from Pepro Tech (Rocky Hill, NJ, USA). Mouse recombinant VEGF, SDF-1 and TGF-β were purchased from R&D Systems (Oxford, UK). Stattic (a STAT3 inhibitor) was purchased from Merck (Darmstadt, Germany). Clodronate (Clod) was purchased from Roche Diagnostics (Indianapolis, IN, USA). Zoledronic acid (ZA) and A83-01 (a TGF-β receptor inhibitor) were purchased from Sigma Chemical Co. (St Louis, MO, USA). Neutralizing antibodies against TGF-β (MAB7346), VEGF (AF-493) and SDF-1 (MAB310) were purchased from R&D Systems. Neutralizing antibody against PDGF-B (ab34074), mCherry (ab125096) antibody and CD68 (ab955) antibody were purchased from Abcam (Cambridge, MA, USA). Neutralizing antibody against MMP-9 (MA5-13595) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). F4/80 antibody (sc-377009) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Recombinant mouse vegf
Manufactured by R&D Systems
Sourced in United Kingdom, United States
Recombinant mouse VEGF is a protein produced in a laboratory setting. It is a member of the vascular endothelial growth factor family and plays a role in the regulation of angiogenesis, the formation of new blood vessels.
Automatically generated - may contain errors
Lab products found in correlation
Stattic, by Merck Group (1 mentions)
Sdf 1, by R&D Systems (1 mentions)
Recombinant human macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions)
Het0016, by Cayman Chemical (1 mentions)
20 hete, by Cayman Chemical (1 mentions)
Recombinant mouse il 4, by Thermo Fisher Scientific (1 mentions)
F4 80 antibody sc 377009, by Santa Cruz Biotechnology (1 mentions)
Zoledronic acid za, by Merck Group (1 mentions)
Il 13, by Thermo Fisher Scientific (1 mentions)
A83 01, by Merck Group (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay kit, by Promega (1 mentions)
Ab33168, by Abcam (1 mentions)
Cell culture supplements, by American Type Culture Collection (1 mentions)
Ab51745, by Abcam (1 mentions)
Ab27526, by Abcam (1 mentions)
Ab7771, by Abcam (1 mentions)
5 protocols using recombinant mouse vegf
1
Macrophage-Targeted Therapy for Angiogenesis
2
Investigating 661W Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse cone photoreceptor-derived 661W cells were kindly provided by Dr. Al-Ubaidi (University of Houston). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from GeneDEPOT (Barker, TX, USA) and HyClone (Logan, UT, USA), respectively. Quercetin, dimethylsulfoxide (DMSO), and bovine serum albumin were obtained from Sigma Aldrich (St. Louis, MO, USA). CellTiter96 AQueous One Solution Cell Proliferation assay kit was purchased from Promega (Madison, WI, USA). Mouse recombinant VEGF was purchased from R&D Systems (Minneapolis, MN, USA). Primary and secondary antibodies used for immunoblotting and immunostaining were described in Table 1 .
3
Endothelial Cell Marker Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture media, serum, and cell culture supplements were purchased from ATCC; Manassas, VA, USA, Millipore; USA, Invitrogen; NY, USA, and PAA; North America. Antibodies against VE‐cadherin (ab33168), Flk‐1 (ab9530), VEGFA (ab51745), STAT3 (ab119351), JAG‐1 (ab7771), NOTCH‐1 (ab27526), SMA; Smooth Muscle Actin (ab5831), and SM22 (ab14106) were purchased from Abcam; Cambridge, UK. The Antibody against GAPDH (sc‐25778) was purchased from Santa Cruz. Antibodies against CD31 were purchased from ABBIOTEC; San Diego, USA (250590) and Santa Cruz; Heidelberg, Germany (sc‐1506). The secondary antibodies for immunostaining anti‐mouse Alexa 488 and anti‐rabbit Alexa 488 were purchased from Invitrogen. The secondary antibodies for Western blotting were purchased from DakoCytomation; Glostrup, Denmark and Abcam. Recombinant mouse VEGF was purchased from R and D Systems; Abingdon, UK.
4
Ovarian Tissue Transplantation with Growth Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biodegradable gels (MedGel, Tokyo, Japan) that were cut into 2 × 2 mm pieces were immersed in 10 μl of 0.1 × phosphate buffered saline (PBS), recombinant mouse VEGF (100 ng/ml, 1 μg/ml, and
10 μg/ml; R&D Systems, Minneapolis, MN, USA), recombinant mouse basic fibroblast growth factor (bFGF; 100 ng/ml, 1 μg/ml, and 10 μg/ml; R&D Systems), or recombinant mouse hepatocyte
growth factor (HGF; 100 ng/ml, 1 μg/ml, and 10 μg/ml; R&D Systems) and then stored in an incubator for 1 h at 37°C. After incubation, biodegradable gels were transplanted into the bursa
of the ovaries of 10-week-old female ICR mice under anesthesia achieved using a mixed anesthetic reagent that contained 60 μg/ml medetomidine, 800 μg/ml midazolam, and 100 μg/ml butorphanol
[17 (link)]. The mixed anesthetic reagent was administered at 50 μl/10 g body weight. A biodegradable gel containing 0.1 × PBS was transplanted into the
bursa of the left ovary, and a gel containing recombinant VEGF, bFGF, or HGF was transplanted into the right ovary. The surgical procedure was performed according to a previous report [18 (link)]. Ovaries from non-treated 10-week-old female ICR mice were used as controls. Ovaries were removed 5 days after transplantation and fixed with SUPER FIX
(KURABO, Osaka, Japan) at 4°C overnight. Then, fixed ovaries were embedded in paraffin and were sliced into 5-μm-thick serial sections.
10 μg/ml; R&D Systems, Minneapolis, MN, USA), recombinant mouse basic fibroblast growth factor (bFGF; 100 ng/ml, 1 μg/ml, and 10 μg/ml; R&D Systems), or recombinant mouse hepatocyte
growth factor (HGF; 100 ng/ml, 1 μg/ml, and 10 μg/ml; R&D Systems) and then stored in an incubator for 1 h at 37°C. After incubation, biodegradable gels were transplanted into the bursa
of the ovaries of 10-week-old female ICR mice under anesthesia achieved using a mixed anesthetic reagent that contained 60 μg/ml medetomidine, 800 μg/ml midazolam, and 100 μg/ml butorphanol
[17 (link)]. The mixed anesthetic reagent was administered at 50 μl/10 g body weight. A biodegradable gel containing 0.1 × PBS was transplanted into the
bursa of the left ovary, and a gel containing recombinant VEGF, bFGF, or HGF was transplanted into the right ovary. The surgical procedure was performed according to a previous report [18 (link)]. Ovaries from non-treated 10-week-old female ICR mice were used as controls. Ovaries were removed 5 days after transplantation and fixed with SUPER FIX
(KURABO, Osaka, Japan) at 4°C overnight. Then, fixed ovaries were embedded in paraffin and were sliced into 5-μm-thick serial sections.
5
Investigating FVIIa and APC Signaling
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!