Macs streptavidin conjugated microbeads

Manufactured by Miltenyi Biotec

MACS streptavidin conjugated microbeads are magnetic particles designed for the isolation and enrichment of biotinylated cells, proteins, or other biomolecules. They provide a simple and efficient method for the separation of labeled target molecules from complex samples.

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Lab products found in correlation

Magmax 96 total rna isolation kit, by Thermo Fisher Scientific (1 mentions) Facsaria fusion sorter, by BD (1 mentions) Ls column, by Miltenyi Biotec (1 mentions) Macs separator, by Miltenyi Biotec (1 mentions) Anti ter119, by BD (1 mentions)

Spelling variants of this product

Streptavidin microbeads (158 citations) MACS microbeads (116 citations) Streptavidin-conjugated microbeads (24 citations) MACS streptavidin-microbeads (9 citations)

2 protocols using macs streptavidin conjugated microbeads

Stem Cell Sorting from Bone Marrow

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For stem cell sorting, BM cells were harvested from femurs, tibiae, humeri and ilia, and incubated with the described biotin-conjugated antibodies against lineage-committed cells. Subsequently, the cells were incubated with MACS streptavidin conjugated microbeads (Miltenyi) for 30 minutes at 4°C. A depletion of the lineage-committed cells was performed using MACS magnets with LS Columns (Miltenyi, #130-042-401). The remaining cells were incubated with the above described antibodies for LKS/HSCs/MPPs. DAPI (0.005μg) was added for viability staining to 10x10⁶ cells right before sorting on a BD FACSAria Fusion sorter. Cells were sorted in 200μl lysis buffer of Magmax-96 Total RNA Isolation Kit (life technologies, Ambion 1830) at room temperature or in PBS containing 0.04% BSA for single cell sequencing. RNA samples were vortexed for 30 s, and subsequently stored at –80°C until RNA extraction.

Khan N., Downey J., Sanz J., Kaufmann E., Blankenhaus B., Pacis A., Pernet E., Ahmed E., Cardoso S., Nijnik A., Mazer B., Sassetti C., Behr M.A., Soares M.P., Barreiro L.B, & Divangahi M. (2020). M. tuberculosis Reprograms Hematopoietic Stem Cells to Limit Myelopoiesis and Impair Trained Immunity. Cell, 183(3), 752-770.e22.

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Isolation of Hematopoietic Stem Cells

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Bone marrow cells were incubated with biotin-conjugated antibodies (all BD Bioscience): anti-Ter-119, anti-CD11b (clone M1/70), anti-CD5 (clone 53–7.3), anti-CD4 (clone RM4–5), anti-CD8a (clone 53–6.7), anti-CD45R (clone RA3–6B2), and anti-Ly6G/C (clone RB6–8C5). Antibodies were added at a concentration of 1:100 for 30 minutes at 4 °C. Cells were subsequently washed with PBS containing 0.5% BSA and incubated with MACS streptavidin-conjugated microbeads (Miltenyi) for 30 minutes at 4 °C. Depleting the lineage-committed cells was performed by magnetic bead separation using LS columns and MACS^® Separator (both Miltenyi).

Priem B., van Leent M.M., Teunissen A.J., Sofias A.M., Mourits V.P., Willemsen L., Klein E.D., Oosterwijk R.S., Meerwaldt A.E., Munitz J., Prévot G., Verschuur A.V., Nauta S.A., van Leeuwen E.M., Fisher E.L., de Jong K.A., Zhao Y., Toner Y.C., Soultanidis G., Calcagno C., Bomans P.H., Friedrich H., Sommerdijk N.A., Reiner T., Duivenvoorden R., Zupančič E., Di Martino J.S., Kluza E., Rashidian M., Ploegh H.L., Dijkhuizen R.M., Hak S., Pérez-Medina C., Bravo-Cordero J.J., de Winther M.P., Joosten L.A., van Elsas A., Fayad Z.A., Rialdi A., Torre D., Guccione E., Ochando J., Netea M.G., Griffioen A.W, & Mulder W.J. (2020). Trained immunity-promoting nanobiologic therapy suppresses tumor growth and potentiates checkpoint inhibition. Cell, 183(3), 786-801.e19.

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