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Npfr mutants are transgenic Drosophila lines that have a disruption in the Neuropeptide F receptor (Npfr) gene. The Npfr gene encodes a G protein-coupled receptor that binds the Neuropeptide F (NPF) neuropeptide, which is involved in the regulation of feeding behavior and other physiological processes in Drosophila. These mutant lines can be used to study the role of the Npfr in various biological functions.

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Lab products found in correlation

Uas trpa1, by Bloomington Drosophila Stock Center (2 mentions) Mb461b, by Bloomington Drosophila Stock Center (2 mentions) Mb370b, by Bloomington Drosophila Stock Center (2 mentions) Mb504b, by Bloomington Drosophila Stock Center (2 mentions) Mb296b, by Bloomington Drosophila Stock Center (2 mentions) Mb320c, by Bloomington Drosophila Stock Center (2 mentions) Mb077b, by Bloomington Drosophila Stock Center (2 mentions) Mb112c, by Bloomington Drosophila Stock Center (2 mentions) Uas npfr rnai, by Vienna Drosophila Resource Center (2 mentions) Vt50658, by Vienna Drosophila Resource Center (2 mentions) Uas npf rnai, by Vienna Drosophila Resource Center (2 mentions) Npf gal4, by Bloomington Drosophila Stock Center (2 mentions)

2 protocols using npfr mutants

1

Fly Behavioral Assay Protocols

Cited 2 times
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Flies were raised at 25°C and 60% relative humidity on standard cornmeal fly food under 12:12h light:dark cycle. 4–7 days old flies were used for experiments and were transferred to fresh food vials 48h before behavioral tests. Fly population was randomized but was kept age matched in each trial. For food deprivation, flies were kept in empty bottles with a wet cotton plug to prevent desiccation. The following fly lines were ordered from Bloomington stock center: Npf-Gal4 (25681), npfr mutants (10747), 20XUAS-TTS-shi[ts1]-p10 (66600; referred to as UAS-shibirets1 in the text), UAS-TrpA1 (26263), MB461B (68327), MB370B (68319), MB504B (68329), MB296B (68308), MB320C (68253), MB077B (68283) and MB112C (68263). UAS-npf-RNAi (108772), UAS-npfr-RNAi (107663) and VT50658 (200166) were ordered from the Vienna Drosophila Resource Center. Other fly lines were described previously: redeye6 (link), R26E0115 (link), R35B1216 , and UAS-CaLexA17 (link). The background control line was Canton-S (Heisenberg) strain.
Chouhan N.S., Griffith L.C., Haynes P, & Sehgal A. (2020). Availability of food determines the need for sleep in memory consolidation. Nature, 589(7843), 582-585.
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2

Fly Behavioral Assay Protocols

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were raised at 25°C and 60% relative humidity on standard cornmeal fly food under 12:12h light:dark cycle. 4–7 days old flies were used for experiments and were transferred to fresh food vials 48h before behavioral tests. Fly population was randomized but was kept age matched in each trial. For food deprivation, flies were kept in empty bottles with a wet cotton plug to prevent desiccation. The following fly lines were ordered from Bloomington stock center: Npf-Gal4 (25681), npfr mutants (10747), 20XUAS-TTS-shi[ts1]-p10 (66600; referred to as UAS-shibirets1 in the text), UAS-TrpA1 (26263), MB461B (68327), MB370B (68319), MB504B (68329), MB296B (68308), MB320C (68253), MB077B (68283) and MB112C (68263). UAS-npf-RNAi (108772), UAS-npfr-RNAi (107663) and VT50658 (200166) were ordered from the Vienna Drosophila Resource Center. Other fly lines were described previously: redeye6 (link), R26E0115 (link), R35B1216 , and UAS-CaLexA17 (link). The background control line was Canton-S (Heisenberg) strain.
Chouhan N.S., Griffith L.C., Haynes P, & Sehgal A. (2020). Availability of food determines the need for sleep in memory consolidation. Nature, 589(7843), 582-585.
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