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Black poly d lysine coated 384 well plates

Manufactured by Greiner
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Sourced in United Kingdom

Black poly-d-lysine-coated 384-well plates are microplates designed for cell culture and biological assays. The plates have a 384-well format and a black polystyrene base coated with poly-d-lysine, a commonly used cell adhesion and attachment surface.

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Lab products found in correlation

Probenecid, by Thermo Fisher Scientific (2 mentions) Flipr tetra, by Molecular Devices (2 mentions) Fluo 4 nw, by Thermo Fisher Scientific (2 mentions)

2 protocols using black poly d lysine coated 384 well plates

1

GLP1R-mediated Calcium Signaling Assay

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CHO cells overexpressing human GLP1R were cultured in black poly-d-lysine-coated 384-well plates (15,000 cells/well; Greiner Bio-One, Stonehouse, UK) at 37°C overnight. Cells were washed with assay buffer, loaded with Fluo-4 NW containing 2.5 mmol/l probenecid (Thermo Fisher Scientific) for 30 min (37°C) and 15 min (room temperature), and then incubated with antibody for 15 min at room temperature before adding GLP-1. Fluorescence was recorded using FLIPR Tetra (Molecular Devices, Wokingham, UK) every 0.5 s for 1 min after agonist addition, followed by every 3 s for a further 4 min. Individual responses were normalised to vehicle control, and average responses were calculated by subtracting the basal fluorescence from the peak intensity. Statistical significance was assessed by one-way ANOVA with post hoc Bonferroni test.
Biggs E.K., Liang L., Naylor J., Madalli S., Collier R., Coghlan M.P., Baker D.J., Hornigold D.C., Ravn P., Reimann F, & Gribble F.M. (2017). Development and characterisation of a novel glucagon like peptide-1 receptor antibody. Diabetologia, 61(3), 711-721.
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2

GLP-1 Receptor Activation Assay in CHO Cells

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CHO cells overexpressing human GLP1R were cultured in black poly-D-lysine-coated 384-well plates (15,000 cells/well; Greiner Bio-One, Stonehouse, UK) at 37°C overnight. Cells were washed with assay buffer, loaded with Fluo-4 NW containing 2.5 mmol/l probenecid (Thermo Fisher Scientific) for 30 min (37°C) and 15 min (room temperature), and then incubated with antibody for 15 min at room temperature before adding GLP-1. Fluorescence was recorded using FLIPR Tetra (Molecular Devices, Wokingham, UK) every 0.5 s for 1 min after agonist addition, followed by every 3 s for a further 4 min. Individual responses were normalised to vehicle control, and average responses were calculated by subtracting the basal fluorescence from the peak intensity. Statistical significance was assessed by one-way ANOVA with post hoc Bonferroni test.
Biggs E.K., Liang L., Naylor J., Madalli S., Collier R., Coghlan M.P., Baker D.J., Hornigold D.C., Ravn P., Reimann F, & Gribble F.M. (2017). Development and characterisation of a novel glucagon like peptide-1 receptor antibody. Diabetologia, 61(3), 711-721.
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