Biotin dutp

Manufactured by Thermo Fisher Scientific

Sourced in United States

Biotin-dUTP is a modified nucleotide that can be incorporated into DNA during synthesis. It contains a biotin molecule attached to the uracil base, which can be detected using avidin or streptavidin-based detection systems.

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Lab products found in correlation

Texas red 12 dutp, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 5 dutp, by Thermo Fisher Scientific (1 mentions) Cy3 dutp, by GE Healthcare (1 mentions) Optical microscope, by Leica (1 mentions) Tdt buffer, by Thermo Fisher Scientific (1 mentions)

2 protocols using biotin dutp

Chromosomal DNA Probe Labeling and FISH

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Spreading of mitotic chromosomes was carried out according to Hoang^{29 (link)}. 5S rDNA, 18S and 26S rDNA probes were generated from S. polyrhiza and from S. intermedia genomic DNA each by using designed primer pairs^{16 (link),21 (link),65 (link),66 (link)} as described^{24 (link)}. Ribosomal DNA, A. thaliana type telomere and S. polyrhiza BAC probes were labeled with Cy3-dUTP (GE Healthcare Life Science), Alexa Fluor 488-5-dUTP, Texas red-12-dUTP, biotin-dUTP or digoxigenin-dUTP (Life Technologies) and precipitated as described^{29 (link)}.
Denaturation of mitotic chromosomes and probes, hybridization, post-hybridization washing and signal detection were carried out according to Lysak et al.^{67 (link)}. Probe stripping and re-hybridization were done as described^{29 (link)}.

Hoang P.T., Fiebig A., Novák P., Macas J., Cao H.X., Stepanenko A., Chen G., Borisjuk N., Scholz U, & Schubert I. (2020). Chromosome-scale genome assembly for the duckweed Spirodela intermedia, integrating cytogenetic maps, PacBio and Oxford Nanopore libraries. Scientific Reports, 10, 19230.

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TUNEL Assay for Apoptosis Detection

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Fixed frozen brain sections were permeabilized in 0.1% Triton X-100 for 15 minutes. After washing in PBS, sections were incubated at 37°C for 1 hour in a 50-μL solution containing 1× TdT buffer (catalog No. 16314015; Life Technologies, Rockville, MD, USA), 11.1 μM biotin-dUTP (catalog No. B32766; Life Technologies), and 20 U of TdT (Life Technologies). The sections were blocked with 2% bovine serum albumin in PBS, incubated in streptavidin-horseradish peroxidase (1:4000; Haoran Biotech Co., Shanghai, China) for 30 minutes, and stained with 3,3′-diaminobenzidine (1:9; Beijing Zhongshan Jinqiao Co., Beijing, China) for 15 minutes. The coverslips were then mounted using mounting medium and examined under an optical microscope (Leica, Oskar-Barnack-Straße, Germany).

Zhang Z., Yang J.L., Zhang L.L., Chen Z.Z., Chen J.O., Cao Y.G., Qu M., Lin X.D., Ji X.M, & Han Z. (2018). 2-(2-Benzofuranyl)-2-imidazoline treatment within 5 hours after cerebral ischemia/reperfusion protects the brain. Neural Regeneration Research, 13(12), 2111-2118.

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