All chemicals were purchased from Sigma Aldrich and cell culture reagents were from Thermo Fischer, unless indicated otherwise. Lipopolysaccharide (E. coli O111:B4), sialidase from V. cholerea, sialyltransferase inhibitor 3-Fax-peracetyl Neu5Ac, vacuolin-1, GW4869, FITC-labeled peanut agglutinin were from Sigma. Anti-Neu1 antibody (clone F8) was from Santa Cruz, anti-PPCA antibody was from Proteintech, anti-Lamp1 antibody (clone 1D4B) was from Biolegend, anti-MAP2 antibody (PA5-17646) was from Thermo Fischer, anti-FLAG (M2) was from Sigma. Protein G agarose, protein A/G magnetic beads, siRNAs and LysoTracker Green DND-26 dye were from Thermo Fischer. Gal-3 was a kind gift from Tomas Deierborg.
Lipopolysaccharide e coli o111 b4
Manufactured by Merck Group
Sourced in Sao Tome and Principe
Lipopolysaccharide (E. coli O111:B4) is a laboratory product derived from the cell walls of the Escherichia coli O111:B4 strain. It is a complex molecule consisting of a lipid and a polysaccharide component, which is commonly used in research and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Sialyltransferase inhibitor 3 fax peracetyl neu5ac, by Merck Group (1 mentions)
Vacuolin 1, by Merck Group (1 mentions)
Anti neu1 antibody clone f8, by Santa Cruz Biotechnology (1 mentions)
Lysotracker green dnd 26 dye, by Thermo Fisher Scientific (1 mentions)
Gw4869, by Merck Group (1 mentions)
Protein g agarose, by Thermo Fisher Scientific (1 mentions)
Sirnas, by Thermo Fisher Scientific (1 mentions)
Protein a g magnetic bead, by Thermo Fisher Scientific (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
Azd5904, by MedChemExpress (1 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Mitoq, by Merck Group (1 mentions)
L name, by Merck Group (1 mentions)
L 012, by Fujifilm (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Luminol, by Merck Group (1 mentions)
Ginsenoside rg1, by Chengdu Biopurify Phytochemicals (1 mentions)
3 protocols using lipopolysaccharide e coli o111 b4
1
Sialidase Regulation of Lysosomal Function
2
Investigating Oxidative Stress Pathways
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MPO inhibitor AZD5904, iNOS inhibitor 1400W (MedChemExpress); mitochondria-targeted antioxidant MitoQ (Merck); NOS inhibitor l -NAME, flavoenzyme inhibitor diphenyleneiodonium chloride (DPI), lipopolysaccharide E. coli O111:B4, phorbol 12-myristate 13-acetate (PMA), luminol (all Sigma-Aldrich). L-012 (Fujifilm Wako Chemicals), Hanks’ Balanced Salt solution and phosphate-buffered saline with/without calcium and magnesium (Gibco). The boronate derivative of fluorescein, FlBA, was synthesized as described previously ([14 ]).
3
Ginsenoside Rg1 Purity and LPS Source
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Ginsenoside Rg1 extracted from the root of P. ginseng was purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). The purity of Rg1 was >99.7%, as determined by HPLC; the chemical structure is shown in Figure 1. Lipopolysaccharide (E. coli O111:B4) was purchased from Sigma Chemical Co. (St. Louis, MO).
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