Pg212

Manufactured by Ipsen

Sourced in China

The PG212 is a high-precision laboratory balance that allows for accurate and reliable measurements of samples. It features a robust construction and advanced weighing technology to ensure consistent and precise results.

Automatically generated - may contain errors

Lab products found in correlation

Enhanced chemi luminescence (ecl), by Merck Group (2 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Bca kit, by Beyotime (1 mentions) Polyvinylidene fluoride (pvdf), by Bio-Rad (1 mentions) P iκbα, by Cell Signaling Technology (1 mentions) Ikkα β, by Santa Cruz Biotechnology (1 mentions) Ab134953, by Abcam (1 mentions) Ab32058, by Abcam (1 mentions) Ab178872, by Abcam (1 mentions) Sc 52746, by Santa Cruz Biotechnology (1 mentions) Sc 52012, by Santa Cruz Biotechnology (1 mentions) P ikkα β, by Cell Signaling Technology (1 mentions) Bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions) A8020, by Solarbio (1 mentions) St505, by Beyotime (1 mentions) Goat anti rabbit igg, by Abcam (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Ab209232, by Abcam (1 mentions) Et1602 18, by Huabio (1 mentions) Ab216776, by Abcam (1 mentions)

4 protocols using pg212

Western Blot Analysis of Inflammatory Markers

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Protein from microglia or ischemic penumbra was centrifuged at 13,000 × g at 4°C for 30 min and quantified through a BCA kit (P0011, Beyotime Biotech). Equal amounts of protein were loaded onto 10% SDS‐PAGE gel (PG212, Epizyme), separated by electrophoresis and transferred to Polyvinylidene fluoride membranes (PVDF, 1620177, Bio‐Rad). The membrane was blocked by 5% non‐fat milk for 2 h and incubated with primary antibodies against IL‐1β (sc52012, Santa Cruz), IL‐6 (sc‐28343, Santa Cruz), TNF‐α (sc‐52746, Santa Cruz), iNOS (BS40374, Bioworld Biotechnology), p‐NF‐κB/p‐P65 (3033, Cell Signaling Technology), NF‐κB/P65 (8242, Cell Signaling Technology), IKKα/β (sc‐8032, Santa Cruz), p‐IKKα/β (2697, Cell Signaling Technology), IκBα (BS3601, Bioworld Biotechnology), p‐IκBα (2859, Cell Signaling Technology), NEMO (ab178872, abcam), anti‐Ubiquitin (ab134953, abcam), anti‐SUMO (ab32058, abcam), COX‐2 (BS1076, Bioworld Biotechnology) or β‐actin (BS40736, Bioworld Biotechnology) overnight at 4°C. On the following day, the membrane was immersed in the corresponding secondary antibodies (BS22357/BS22356, Bioworld Biotechnology) on the shaker for 1–2 h. After moistened with the enhanced chemiluminescence (ECL, 34580, Thermo Fisher Scientific), the protein band was visualized through Gel‐Pro system (Tanon Technologies) and analyzed using ImageJ (ImageJ 1.5, NIH).

Lan Z., Qu L., Liang Y., Chen L., Xu S., Ge J., Xue Z., Bao X., Xia S., Yang H., Huang J., Xu Y, & Zhu X. (2024). AZD1390, an ataxia telangiectasia mutated inhibitor, attenuates microglia‐mediated neuroinflammation and ischemic brain injury. CNS Neuroscience & Therapeutics, 30(4), e14696.

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Crosslinking Gal-8 and LILRB4 Proteins

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Recombinant Gal-8 and LILRB4-his protein (16742-H08H, Sino Biological) were mixed in a 1:1 ratio of moles at 37°C for 30 min and incubated for 5 min at RT with Glutaric dialdehyde of indicated concentrations. The samples were then electrophoresed in SDS-PAGE (PG212, Epizyme) and silver-stained (P0017S, Beyotime).

Wang Y., Sun Y., Deng S., Liu J., Yu J., Chi H., Han X., Zhang Y., Shi J., Wang Y., Quan Y., Li H, & Xu J. (2024). Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors. Cell Reports Medicine, 5(1), 101374.

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Protein Extraction and Western Blot Analysis

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Cells were lysed using RIPA buffer containing 1% Phenylmethanesulfonyl fluoride (PMSF, ST505, Beyotime, Shanghai, China) and 2% phosphatase inhibitor. The total protein concentration was quantified using the Bicinchoninic Acid Protein Assay Kit (ST505, Thermo Fisher Scientific, Waltham, MA, United States). Next, protein samples (30 mg) were separated using 10% SDS-PAGE (PG212, EpiZyme, Shanghai, China). Then, the proteins were transferred to PVDF membranes, followed by blocking with 5% BSA (A8020, Solarbio, Beijing, China) at room temperature for 1 h. Afterwards, the membranes were incubated with primary antibodies at 4°C overnight. Subsequently, the membranes were washed with PBST and incubated with a goat anti-mouse or goat anti-rabbit secondary antibody for 1 h at room temperature. Finally, the protein bands were visualized with ECL (Millipore) and quantified with ImageJ software (National Institutes of Health) the manufacturer’s instructions.

Liu C.X., Gao Y., Xu X.F., Jin X., Zhang Y., Xu Q., Ding H.X., Li B.J., Du F.K., Li L.C., Zhong M.W., Zhu J.K, & Zhang G.Y. (2024). Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells. World Journal of Gastroenterology, 30(5), 485-498.

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Cardiac Protein Extraction and Western Blot

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Total protein was extracted from frozen heart tissue using a Minute™ muscle tissue total protein extraction kit (SA-06-MS, Invent Biotechnologies, Inc., United States) following the manufacturer’s instructions. Protein samples were quantified using a BCA Protein Assay Kit (E-BC-K318-M, Elabscience, Wuhan, China). Protein samples were resolved on 10% SDS-PAGE gels (PG212, EpiZyme, Shanghai, China) and transferred onto polyvinylidene fluoride membranes (Millipore, Burlington, MA, United States). The membranes were blocked with 5% fat-free milk and incubated overnight at 4°C with primary antibodies (ANP, 1:1000, ab209232, Abcam, United States; β-MHC, 1:1000, 22280-1-AP, Proteintech, China; p38, 1:1000, ET1702-65, Huabio, China; JNK, 1:1000, RT1550, Huabio, China; ERK1/2, 1:1000, 67170-1-Ig, Proteintech, China; p-p38, 1:1000, ER1903-01, Huabio, China; p-JNK, 1:1000, 4668S, Cell Signaling Technology, USA; p-ERK1/2, 1:1000, 4370T, Cell Signaling Technology, United States; DUSP 6, 1:1000, ET1602-18, Huabio, China; β-ACTIN, 1:1000, ab8226, Abcam, United States). Then, the membranes were washed and incubated with secondary antibodies (Goat anti-Mouse IgG, 1:10000, ab216776, Abcam; Goat Anti-Rabbit IgG, 1:10000, ab6721, Abcam). The protein bands were visualized by ECL (Millipore) and quantified using ImageJ software (National Institutes of Health).

Xu Q., Ding H., Li S., Dong S., Li L., Shi B., Zhong M, & Zhang G. (2021). Sleeve Gastrectomy Ameliorates Diabetes-Induced Cardiac Hypertrophy Correlates With the MAPK Signaling Pathway. Frontiers in Physiology, 12, 785799.

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