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The M-112 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating samples based on their density differences. The M-112 can accommodate a variety of sample tubes and microplates, enabling efficient sample processing.

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Lab products found in correlation

H 300, by Santa Cruz Biotechnology (3 mentions) Chemidoc system, by Bio-Rad (2 mentions) Ab176840, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Anti hif 2α, by Cell Signaling Technology (1 mentions) Anti hif 1β, by Cell Signaling Technology (1 mentions) Anti daxx, by Santa Cruz Biotechnology (1 mentions) Anti occludin, by Proteintech (1 mentions) Ac 15, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Anti ha, by Fortrea (1 mentions) Anti β actin, by Merck Group (1 mentions) Ha 11, by Fortrea (1 mentions) Anti e cadherin, by BD (1 mentions) Anti n cadherin, by BD (1 mentions) Anti hif 1α, by BD (1 mentions) Anti vimentin, by BD (1 mentions) Anti lamin a c 4c11, by Cell Signaling Technology (1 mentions) Actin, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

4 protocols using m 112

1

Quantifying Chromatin Protein Levels

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Protein extracts were separated by SDS–polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and probed with antibodies against Daxx (M-112, Santa Cruz Biotechnology, 1:500), Atrx (H-300, Santa Cruz Biotechnology, 1:1,000), β-actin (A1978, Sigma, 1:5000) and H3.3 (Ab176840, Abcam, 1:1000). Proteins were visualized using Li-COR secondary antibodies and imaged using a ChemiDoc System (Bio-Rad). Protein levels were quantified in ImageJ and normalized to the level in the first lane.
Sun C., Qi Y., Fowlkes N., Lazic N., Su X., Lozano G, & Wasylishen A.R. (2023). The histone chaperone function of Daxx is dispensable for embryonic development. Cell Death & Disease, 14(8), 565.
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2

Immunoblotting Antibody Panel for EMT Analysis

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Primary antibodies used for immunoblotting were as follows: monoclonal anti-HA (1:5,000; HA11; Covance), anti-Flag (1:5,000; M2; Sigma Aldrich), anti-Daxx (1:2,000, M112; Santa Cruz Biotechnology), anti-E-cadherin (1:1,000; 610182, BD Biosciences), anti-occludin (1:2,000; 13409, Proteintech), anti-vimentin (1:10,000; 550513, BD Biosciences), anti-N-cadherin (1:1,000; 610921, BD Biosciences), polyclonal anti-Slug (1:1,000; G18, Santa Cruz Biotechnology), anti-β-actin (1:10,000; AC-15, Sigma Aldrich), anti-HIF-1α (1:2,000; 610958, BD Biosciences), anti-HIF-2α (1:1,000; 7096, Cell Signaling) and anti-HIF-1β (1:2,000; 5537, Cell Signaling).
Lin C.W., Wang L.K., Wang S.P., Chang Y.L., Wu Y.Y., Chen H.Y., Hsiao T.H., Lai W.Y., Lu H.H., Chang Y.H., Yang S.C., Lin M.W., Chen C.Y., Hong T.M, & Yang P.C. (2016). Daxx inhibits hypoxia-induced lung cancer cell metastasis by suppressing the HIF-1α/HDAC1/Slug axis. Nature Communications, 7, 13867.
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3

ATRX, DAXX, and Lamin A/C Detection

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Cells were lysed with NETN lysis buffer (100 mM NaCl, 0.5 mM EDTA, 50 mM Tris–HCl, pH 8.0 and 0.5% NP-40) with protease inhibitor cocktail (Roche cOmplete, EDTA-free). The protein samples were separated by SDS-PAGE gel, and then transferred to nitrocellulose membrane. The following antibodies were used; anti-ATRX antibody (H-300 Santa Cruz Biotechnology, A301-045A Bethyl Laboratories), DAXX (M-112 Santa Cruz Biotechnology), Anti-Lamin A/C (4C11 Cell Signaling), and Actin (A1978 Sigma-Aldrich).
Min J., Wright W.E, & Shay J.W. (2017). Alternative lengthening of telomeres can be maintained by preferential elongation of lagging strands. Nucleic Acids Research, 45(5), 2615-2628.
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4

Western Blot Analysis of Chromatin Regulators

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Tissue samples were flash frozen, pulverized and lysed in sodium dodecyl sulfate (SDS) lysis buffer (1% SDS, 6.5 mM Tris-HCl pH 6.8, 25% glycerol, 10% β-mercaptoethanol). Protein extracts were separated by SDS/polyacrylamide gel electrophoresis (PAGE), transferred to nitrocellulose membranes, and probed with antibodies against Daxx (M-112, Santa Cruz Biotechnology, 1:1000), Atrx (H-300, Santa Cruz Biotechnology, 1:500), Men1 (A300-105A, Bethyl Laboratories, 1:1000), vinculin (V9131, Millipore Sigma, 1:1000) and actin (12004163, Bio-Rad, 1:5000). Proteins were visualized using Li-COR secondary antibodies and imaged using a ChemiDoc System (Bio-Rad). Signal was quantified using ImageJ software (NIH).
Sun C., Estrella J.S., Whitley E.M., Chau G.P., Lozano G, & Wasylishen A.R. (2022). Context matters – Daxx and Atrx are not robust tumor suppressors in the murine endocrine pancreas. Disease Models & Mechanisms, 15(8), dmm049552.
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