Pcdna4 v5 hisa vector

Manufactured by Thermo Fisher Scientific

The PcDNA4 V5-HisA vector is a mammalian expression vector designed for the production and detection of recombinant proteins in eukaryotic cells. It features a cytomegalovirus (CMV) promoter for strong, constitutive expression, a C-terminal V5 epitope tag, and a C-terminal polyhistidine (6xHis) tag for purification and detection of the expressed protein.

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Lab products found in correlation

Pcdna3 ha vector, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem medium, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Zeocin, by InvivoGen (1 mentions) Jetprime, by Polyplus Transfection (1 mentions) Hek293t 17 cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

PcDNA4/V5 (2 citations)

2 protocols using pcdna4 v5 hisa vector

HCV Genome and Protein Expression

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pUC-JFH1 containing full length 2a type HCV genome is a kind gift from T. Wakita. E2 (amino acids 384-750 of JFH1) was cloned into pcDNA3-HA vector (Invitrogen) with N-terminal HA tag or pCMV-tag 2B vector (Stratagene) with N-terminal FLAG tag. HCV E2, core-E1 and core-E1-E2 were separately cloned into pcDNA4 V5-HisA vector (Invitrogen) with C-terminal V5 tag. Soluble fraction of 1b type (H77) E2 (amino acids 384-661) was pEF6A-V5-6XHis vector for proximity ligation assay.

Kim M.S., Kim S, & Myung H. (2014). Degradation of AIMP1/p43 Induced by Hepatitis C Virus E2 Leads to Upregulation of TGF-β Signaling and Increase in Surface Expression of gp96. PLoS ONE, 9(5), e96302.

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Stable PSMA Expression in HEK293T Cells

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HEK293T/17 cells obtained from the American Type Culture Collection were grown in DMEM medium (Sigma–Aldrich, Steinheim, Germany) supplemented with 10% v/v fetal bovine serum (FBS; Gibco, Life Technologies, Carlsbad, CA). PC-3 and DU145 cells, also from the American Type Culture Collection, LNCaP cells, kindly provided by Z. Hodny (IMG, Prague, Czech Republic), and CW22Rv1 cells, a gift from R. Lapidus (UMBC, Baltimore, MD), were all grown in RPMI 1640 medium (Sigma–Aldrich) with 10% v/v FBS. All cell lines were kept under humidified 5% CO₂ atmosphere at 37°C. Stable overexpression of human PSMA in HEK293T/17 cells was realized by transfection of pcDNA4/V5-His A vector (Invitrogen, Carlsbad, CA) carrying the cDNA for full-length human PSMA (FOLH1; NCBI Reference sequence: NM_004476.1). Following transfection using jetPRIME (Polyplus-transfection, Illkirch, France), cells were selected in the presence of Zeocin (25 μg/ml; InvivoGen, San Diego). Stable transfectants were isolated by repeated cloning of single cell progeny.

Nováková Z., Foss C.A., Copeland B.T., Morath V., Baranová P., Havlínová B., Skerra A., Pomper M.G, & Barinka C. (2017). Novel Monoclonal Antibodies Recognizing Human Prostate-Specific Membrane Antigen (PSMA) as Research and Theranostic Tools. The Prostate, 77(7), 749-764.

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