TEM imaging
was performed using a JEOL
2100Plus TEM microscope operated at 200
kV. For liquid samples, a drop of solution was placed on Cu grids
coated with a
carbon film (Agar Scientific), stained with 1 wt % uranyl
acetate acid (u.a.; Sigma-Aldrich), and dried. Excess peptide and
staining solutions were blotted using filter paper. TEM grids for
the peptide gel were prepared by placing a small amount of gel on
a TEM grid using a spatula. The excess of gel was removed by blowing
compressed air. The residual gel on the surface of the TEM grid was
stained using 1 wt % u.a. and dried.
Castelletto V, & Hamley I.W. (2022). Amyloid and Hydrogel Formation of a Peptide Sequence from a Coronavirus Spike Protein. ACS Nano, 16(2), 1857-1867.