Sodium salt of cacodylic acid

Commercially lyophilized preparation of holo α-Lactalbumin (from bovine milk) was purchased from Sigma Chemical Co (St Louis, MO, USA). Ficoll 70, trichloroacetic acid (TCA), Thioflavin T (ThT), 8-Anilino-1-naphthalenesulfonic acid ammonium salt (ANS), Ethylenediaminetetraacetic acid (EDTA) and sodium salt of cacodylic acid were also obtained from Sigma Chemical Co. Potassium chloride (KCl) and sodium acetate were obtained from Merck. Calcium chloride was obtained from G-Biosciences. These and other chemicals, which were of analytical grade, were used without further purification.

Fresh got heart was collected from the Slaughter House, Ghazipur Mandi, New Delhi. The cyt-c was isolated from goat heart according to the method described previously [31 (link),32 (link)]. The protein was purified by gel filtration (90×2.3 cm) on Sephadex G-50 column chromatography. The purity was checked using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on which a single band was observed indicating the purity of the protein. The laboratory reagents such as LiCl, LiClO₄, LiBr, ANS and sodium salt of cacodylic acid were purchased from Sigma Chemical Co. (U.S.A.). Sodium chloride and potassium chloride were purchased from Merck (India). These and other chemicals were analytical-grade reagents and were used without further purification.