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289 protocols using tetrahydrofuran thf

1

Synthesis of Lanthanide-based Nanoparticles

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Yttrium (III) acetate hydrate, cerium (III) acetate hydrate, terbium (III) acetate hydrate, gadolinium (III) acetate hydrate (99.9%), oleic acid (OA, 90%), 1-octadecene (ODE, 90%), sodium hydroxide (NaOH), ammonium fluoride (NH4F), ethanol (absolute), methanol, cyclohexane, tetrahydrofuran (THF), dopamine hydrochloride, hydrochloric acid (HCl), peptone, L-cysteine, maltose, sodium phosphate dibasic (Na2HPO4), and tetrahydrofuran (THF, ≥97%) were purchased from Sigma-Aldrich (St Louis, Mo, USA). C. novyi-NT spores were kindly provided by BioMed Valley Discoveries (Kansas City, MO, USA). Lipiodol™ was obtained from Guerbet (Villepinte, France).
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2

Camptothecin-Loaded Poly(Anhydride) Nanocarriers

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Poly(methyl vinyl ether-co-maleic anhydride) or poly(anhydride) (Gantrez™ AN 119; MW 200,000) was purchased from Ashland Inc. (KY, USA). Camptothecin (CPT) (99.0%) was supplied by 21CECpharm (London, UK). 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), methoxy poly(ethylene glycol) 2000 (mPEG), Lumogen® red, DAPI (4′,6-diamidino-2-phenylindole), solid iodine (≥99.8%), pepsin and pancreatin were obtained from Sigma-Aldrich (Germany). Tissue-Tek® OCT compound was purchased from Sakura, The Netherlands. Acetone, ethanol, acetonitrile, formaldehyde, tetrahydrofuran (THF) and trifluoroacetic acid (TFA) were obtained from Merk (Darmstadt, Germany). All other reagents and chemicals used were of analytical grade and supplied from Sigma Aldrich (MO, USA) and Merck (Darmstadt, Germany).
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3

Macrophage Interaction with Stimuli-Responsive Polymers

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Poly(n-isopropylacrylamide) (PNIPAM, Mn 40 kDa), poly(vinyl alcohol) (PVA, 88% hydrolyzed, molecular weight (MW) =3 kDa), fluorescein O-acrylate, 2,2’-azobis(isobutyronitrile) (AIBN), dextran-fluorescein isothiocyanate (dextran-FITC, MW = 3-5 kDa), lipopolysaccharides (LPS) from Escherichia coli O111:B4, and zymosan (zymosan A from saccharomyces cerevisiae) were purchased from MilliporeSigma. n-isopropylacrylamide (NIPAM) was purchased from TCI America. Fetal bovine serum (FBS) was purchased from VWR. 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI), 3,3'-dioctadecyloxacarbocyanine (DiO) and LysoView™ 488 (Lysoview) were purchased from Biotium. Carboxyfluorescein succinimidyl ester (CFSE) was purchased from ThermoFisher. Hoechst 33342 was purchased from Life Technologies. Poly(dimethyl siloxane) (PDMS) kit (Sylgard 184) was purchased from Dow Corning. Poly(n-propyl methacrylate) (PPMA) with a Mw of 150 kDa was purchased from Scientific Polymer Products. RAW264.7 macrophages were purchased from the American Type Culture Collection. Dulbecco’s modified eagle’s medium (DMEM, with 4.5 g/L glucose and 4 mM L-glutamine) was purchased from Lonza Bioscience. Poly(3-hexylthiophene-2,5-diyl) (P3HT, MW = 58 kDa) was purchased from Rieke Metals. Tetrahydrofuran (THF) was purchased from Merk.
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4

Biodegradable PCL-PEI Nanocarriers for Doxorubicin Delivery

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Poly(ε-caprolactone) (PCL) 45 kDa was purchased from Polysciences Asia Pacific, Taiwan, N,N′-dicyclohexylcarbodiimide and dichloromethane were obtained from Merck, Germany. Polyethyleneimine (PEI) 600 Da, glutaraldehyde, Dulbecco's modified eagle medium (DMEM) and tetrahydrofuran (THF) were purchased from Sigma-Aldrich, USA and N-hydroxysuccinimide (NHS), fetal bovine serum (FBS), penicillin/streptomycin and dialysis membranes (5, 10, and 15 kDa MWCO) were obtained from Thermo Fisher Scientific USA. Moreover, deionized water from Merck Millipore, USA, trypsin–EDTA solution from Invitrogen Life Technologies and doxorubicin HCl from LGM Pharma, USA was used. All other materials used in this research work were of analytical grade and used as received without further processing.
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5

Synthesis of Superhydrophobic OPSZ Coatings

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Durazane™ 1500RC (Merck, Germany), consisting of 3-aminopropyltriethoxysilane-substituted polymethyl-(hydro)/polymethylsilazane, was used as the OPSZ precursor (Mw = 1745, PDI = 22), and its molecular structure is illustrated in Scheme 1A. 1H,1H,2H,2H-Perfluorodecyltrimethoxysilane (FAS-17) was obtained from Sooyang Chemtec (Gamgok-myeon, Korea). Toluene (99.8%), tetrahydrofuran (THF, ≥99%), trifluoroToluene (TFT, ≥99%), and hexamethyldisilazane (≥99%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Acetone (99.5%), ethanol (99.5%), and hydrochloric acid (37 wt%) were supplied by Samchun Chemical (Korea). All the chemicals were used as received without further purification.
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6

Solvent Preparation for Chemical Analysis

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Ethanol (96,3% v/v) was supplied by SIA Kalsnavas elevators (Jaunkalsnava, Latvia). Methanol (≥99.8%), hydrochloric acid (HCl) (≥37%), and tetrahydrofuran (THF) (anhydrous, ≥99.9%) were obtained from Sigma-Aldrich (Steinheim, Germany). Isopropanol (Reag. Ph Eur, ≥99.8%) was received from Merck (Darmstadt, Germany). 1-Butanol (99%) was purchased from Acros Organics (Geel, Belgium). Potassium hydroxide (KOH) (Reag. Ph Eur, 85.0–100.5%) was provided by VWR International (Leuven, Belgium). Nitric acid (HNO3) (≥65%) and acetone (≥99%) were obtained from Honeywell (Seelze, Germany).
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7

Graphene-TPU Composite Fabrication

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Graphene (06-0210) was obtained from Strem Chemicals Corporation. TPU (2363-80AE) was purchased from Lubrizol Corporation. Tetrahydrofuran (THF) was obtained from Sigma, United Kingdom. Rat Schwann cells (RSC-96) were purchased from Beijing DingGuo biotechnology Co. Deionized water was used throughout the experiments.
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8

Polypropylene-grafted maleic anhydride synthesis

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Polypropylene-grafted maleic anhydride (PPgMA, average Mn 3900 g/mol, average Mw 9100 g/mol, and 8–10 wt. % maleic anhydride) was purchased from Sigma Aldrich (Munich, Germany).
Polypropylene-grafted maleic anhydride with higher molecular weight produced by Exxon Mobil USA (PPgMASB, Mn 50–100 kg/mol, and 0.5–1 wt. % maleic anhydride) was kindly supplied by SABIC (Geleen, The Netherlands). Analytical-grade furfurylamine (FFA, >99%) was purchased from Sigma Aldrich (Munich, Germany) and was freshly distilled before the experiment. Analytical-grade 1,1′-(methylenedi-4,1-phenylene) bismaleimide (BM, 95%), tetrahydrofuran (THF, >99.9%), and chloroform (CHCl3, >99%) were purchased from Sigma Aldrich (Munich, Germany). 1–2 dichlorobenzene (DCB, >99%) was purchased from Fluka (Landsmeer, The Netherlands). Antioxidant AOB225 (CAS Number 9421-57-8) was kindly supplied by SABIC (Geleen, The Netherlands).
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9

Synthesis of Organic Compounds Using Common Reagents

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Anhydrous dichloromethane (DCM) and tetrahydrofuran (THF) were purchased from Sigma Aldrich. Absolute ethanol was obtained from Decon Laboratories. Diethyl ether (Et2O), methyl tert‐butyl ether (MTBE), and sodium azide were purchased from Fisher Scientific. Triethylamine (NEt3) and methanesulfonyl chloride (MsCl) were obtained from Acros and distilled from CaH2 and P2O5, respectively. Trifluoroacetic acid (TFA) was purchased from Oakwood Products. Pearlman's catalyst (Pd(OH)2/C), 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO), phenylacetylene (PA), diphenylacetylene (DPA), dimethyl acetylenedicarboxylate (DMAD), and diethyl acetylenedicarboxylate (DEBD) were purchased from Sigma Aldrich and used as received. Potassium bromide and di‐tert‐butyl dicarbonate ((Boc)2O) were purchased from Acros and used as received.
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10

Nanoparticle-Based Potentiometric Sensing

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Bis(2-ethylhexyl) sebacate (DOS), chromoionophore II (CHII, 9-Dimethylamino-5-[4-(16-butyl-2,14-dioxo-3,15-dioxaeicosyl)phenylimino]benzo[a]phenoxazine, ETH 2439), potassium ionophore III (KI3, 2-Dodecyl-2-methyl-1,3-propanediyl bis[N-[5′-nitro(benzo-15-crown-5)-4′-yl]carbamate]), Sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaBARF), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), sodium chloride (NaCl), dichloromethane (DCM) and tetrahydrofuran (THF) were purchased from Sigma-Aldrich. Quantum dots were purchased from NN-Labs (emission peak 640 nm, CZ640) and Cytodiagnostics (emission peak 490 nm, FN-490). Tris Base was purchased from Fisher BioReagents. Spectra/Por in vivo micro-dialysis hollow fibers (inner diameter 200 μm; outer diameter 280 μm, MWCO 13 kD) were purchased from Spectrum Labs, Inc. 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-550] (ammonium salt) (DSPE-mPEG550) was purchased from Avanti Polar Lipids, Inc.
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