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Duolink starter kit reagents

Manufactured by Merck Group
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The Duolink starter kit reagents are a set of laboratory tools designed for protein interaction studies. The kit includes all the necessary components for performing in situ proximity ligation assays, which enable the visualization and quantification of protein-protein interactions within cells. The reagents provided in the kit facilitate the detection and analysis of these interactions.

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Lab products found in correlation

Ab133741, by Abcam (2 mentions) Ab10530, by Abcam (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Las x software, by Leica (1 mentions) Ab10261, by Abcam (1 mentions)

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Duolink kit (106 citations) Duolink reagent (2 citations)

2 protocols using duolink starter kit reagents

1

Proximity Ligation Assay for NPM1

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PLA was performed with the Duolink starter kit reagents (DUO92103, Sigma) according to manufacturer's instructions using antibodies against FLAG (goat, ab95045, Abcam) and NPM1 (mouse, ab10530, Abcam). After completion of the PLA protocol, we proceeded with incubation of a third antibody produced in rabbit to detect cellular compartments such as nucleolus with FBL (FIBRILLARIN, rabbit, ab184817 Abcam) or nuclear membrane with LMNB1 (LAMINB1, rabbit, ab133741, Abcam) following the procedure for immunofluorescence. Images were obtained with a Leica SP8 confocal microscope and processed with the LASX software.
Izzo A., Akol I., Villarreal A., Lebel S., Garcia-Miralles M., Cheffer A., Bovio P., Heidrich S, & Vogel T. (2023). Nucleophosmin 1 cooperates with the methyltransferase DOT1L to preserve peri-nucleolar heterochromatin organization by regulating H3K27me3 levels and DNA repeats expression. Epigenetics & Chromatin, 16, 36.
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2

Proximity Ligation Assay for Protein Interactions

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Proximity ligation assay (PLA) was performed with the Duolink starter kit reagents (DUO92103, SIGMA) according to the manufacturer’s instruction as outlined in the Supplementary Material and Methods. The following antibodies were used: anti-Au1 (1:2000, mouse, Covance) and anti-DDX5 (1:200, goat, ab10261, abcam) for PLA and anti-Lamin B1 (1:200, rabbit, ab133741, abcam). Images were taken with a confocal microscope and analysed with the LASX software (SP8, Leica, Jena, Germany).
Weise S.C., Arumugam G., Villarreal A., Videm P., Heidrich S., Nebel N., Dumit V.I., Sananbenesi F., Reimann V., Craske M., Schilling O., Hess W.R., Fischer A., Backofen R, & Vogel T. (2018). FOXG1 Regulates PRKAR2B Transcriptionally and Posttranscriptionally via miR200 in the Adult Hippocampus. Molecular Neurobiology, 56(7), 5188-5201.
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