Qauntifluor ssdna system
The QuantiFluor ssDNA System is a fluorometric assay that provides a sensitive and specific method for the quantification of single-stranded DNA (ssDNA) in solution. The system utilizes a proprietary fluorescent dye that selectively binds to ssDNA, allowing for accurate measurement of ssDNA concentration.
Lab products found in correlation
5 protocols using qauntifluor ssdna system
RNA-seq Library Preparation and Analysis
Genomic DNA Extraction and Sequencing
mRNA Sequencing Library Preparation
Whole Transcriptome Sequencing Library Prep
The double-stranded library was quantified using the QauntiFluor ONE dsDNA System (Promega, Madison, WI, USA) and 330 ng in a total volume of 60 μl or less. The library was cyclized at 37 °C for 60 min, digested at 37 °C for 30 min, and then the circularization product was cleaned up. The library was incubated at 30 °C for 25 min with DNA nanoball (DNB) enzyme. Finally, the library was quantified using the QauntiFluor ssDNA System (Promega) and sequenced using the MGIseq system (MGI) to generate 150 bp paired-end reads.
RNA-seq Library Preparation Protocol
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