To observe cell morphology, cells were stained after 5 days in culture. Cells were fixed with 3.7% paraformaldehyde, permeabilized with Triton X-100 (0.5% in PBS) and blocked (to avoid non-specific staining by the secondary antibody) with a 1% bovine serum albumin solution containing 0.2% Triton X-100. Cells were then immunostained with the primary antibody against vinculin, a focal adhesion protein (Anti-Vinculin antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture, Product Number V9264, Sigma-Aldrich, Burlington, MA, USA) followed by the secondary antibody (Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, Catalog #:A-11001, Molecular 142 Probes, Thermo Fisher Scientific, Waltham, MA, USA). To observe the F-actin cytoskeleton, cells were stained with Acti-stain 555 Phalloidin (100 nM in PBS) (Cat. # PHDH1, Cytoskeleton, Inc., Denver, CO, USA) and to observe nuclei, cells were stained with DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate, Cat # D3571, Invitrogen, Waltham, MA, USA: 300 nM in PBS). All samples were mounted on glass coverslips with fresh PBS and imaged with an epi-fluorescence microscope Nikon Ti-S. The microscopy of the tick film cross-section and the Hap film surface with 0 h of immersion (control) was performed in a TESCAN-Vega3 scanning electron microscope.
Vega 3 scanning electron microscope
Manufactured by TESCAN
Sourced in Czechia
The Vega 3 is a scanning electron microscope (SEM) designed and manufactured by TESCAN. It is capable of producing high-resolution images of samples by scanning them with a focused beam of electrons. The Vega 3 can be used to analyze the surface, structure, and composition of materials at the microscopic level.
Automatically generated - may contain errors
Lab products found in correlation
K550x sputter coater, by Quorum Technologies (6 mentions)
Digimizer, by MedCalc (2 mentions)
Q150r es, by Quorum Technologies (2 mentions)
Dxr raman microscope, by Thermo Fisher Scientific (2 mentions)
Anti vinculin antibody, by Merck Group (1 mentions)
Dimedone, by Merck Group (1 mentions)
Potassium permanganate, by Merck Group (1 mentions)
Phosphorus pentoxide, by Merck Group (1 mentions)
Benzaldehyde, by Merck Group (1 mentions)
Malononitrile, by Merck Group (1 mentions)
3 chloropropyl trimethoxysilane, by Merck Group (1 mentions)
Vector 22 spectrometer, by Bruker (1 mentions)
Graphite powder, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
1 methylimidazole, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
D8 advance diffractometer, by Bruker (1 mentions)
37 protocols using vega 3 scanning electron microscope
1
Visualizing Cell Morphology and Cytoskeleton
2
Graphite-based Hybrid Materials Synthesis
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Graphite powder, 1-methylimidazole (99%), (3-chloropropyl)-trimethoxysilane (97%), iron(iii ) chloride hexahydrate (≥99%), dimedone (95%), malononitrile (≥99%), and ethanol (99%) were all purchased from Sigma-Aldrich. Sulfuric acid (95–98%), phosphorus pentoxide, potassium persulfate, potassium permanganate, hydrogen peroxide (30%), hydrochloric acid (37%), and benzaldehydes (97–99%) were purchased from Merck. Deionized water was distilled by water purification system (Milli-Q System). Fourier transform infrared (FT-IR) spectroscopy was recorded on a Bruker-Vector 22 spectrometer (Germany). Powder X-ray diffraction (PXRD) was obtained using a Bruker D8 ADVANCE diffractometer (Germany). Thermal gravimetric analysis (TGA) was carried out using a Netzsch STA 409 PC/PG apparatus (Germany). The morphology of the particles was characterized by TESCAN-Vega 3 scanning electron microscope (SEM) (Czech Republic). Energy dispersive X-ray (EDX) spectroscopy was obtained by using TESCAN-Vega 3 apparatus (Czech Republic).
3
Scanning Electron Microscopy of Cuticular Wax
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To observe cuticular wax crystalloids, five leaves from each diploid and tetraploid plant under control and drought treatment conditions were collected and air-dried at room temperature. Microscopic observation was performed using a TESCAN-Vega 3 scanning electron microscope (Brno, Czech Republic) [18 (link)].
4
Scanning Electron Microscopy of Venetin-1 Treated Lung Cancer Cells
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After the incubation with Venetin-1, the non-small lung carcinoma A549 cells were observed by SEM. First, they were fixed with 4% glutaraldehyde in 0.1 M phosphate buffer at pH 7.0. Subsequently, the cells were mounted on slides and stained with a 1% OsO4 solution for 1 h, followed by dehydration in a series of acetone solutions (15%, 30%, 50%, 70%, 100%). The preparations were dried in a desiccator using silica gel for 24 h and gold-plated using a K550X coater (Quorum Technologies, United Kingdom). The fixed cells were analyzed using a Tescan Vega 3 scanning electron microscope (Tescan, Czech Republic).
The structure of Venetin-1 was observed and documented with a Quanta 3D FEG scanning electron microscope (FEI, Japan). A lyophilized preparation, which had not been subjected to standard dehydration and gold sputtering, was used for microscopic observations. This procedure allowed visualization of the active substance in its natural shape.
The structure of Venetin-1 was observed and documented with a Quanta 3D FEG scanning electron microscope (FEI, Japan). A lyophilized preparation, which had not been subjected to standard dehydration and gold sputtering, was used for microscopic observations. This procedure allowed visualization of the active substance in its natural shape.
5
Carbon Nanofiber Growth on Graphite Felt
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Graphite felt (GF) (Hunan Jiuhua Carbon High-Tech Co., Xiangtan, Hunan, China) was firstly soaked in 10 wt% FeCl3 for 1 h and then dried in a vacuum oven at 100°C for 1 h. The growth of carbon nanofibers onto GF was conducted in a furnace equipped with a quartz tube. The GF was heated to 850°C at a rate of 5°C/min in N2 atmosphere, then inlet the mixture of H2 and N2 (H2/N2 = 1 : 4) at a total flow of 100 mL min−1 for 1 h to reduce the Fe (III) to Fe (0). Subsequently, let the furnace cool down to about 750°C and then inlet acetylene with rate of 10 mL min−1 for 5 min. After cooling down to room temperature naturally, the CNFs/GF was taken out. The residue Fe in the CNFs/GF was removed by socking it in 0.5 M hydrochloric acid solution and rinsed with distilled water. At last, the samples were dried in the drying oven at 100°C for 1 h. The morphology characterization of samples was observed by a Tescan Vega-3 scanning electron microscope (SEM).
6
Comprehensive Characterization of Crystalline Materials
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The crystal phase and crystallinity of samples were studied by the X-ray diffraction (XRD) method (Rigaku SmartLab 3 diffractometer (Tokyo, Japan) of the engineering center of the Saint Petersburg State Technological Institute (Technical University)) using Cu-Kα irradiation (λ = 1.54 Å). Samples were scanned along 2θ in the range of 10–70° at 0.5 degrees/min. For XRD analysis, samples were dried at 120 °C for 4 h. For SEM analysis, the samples were dried in vacuo for 2 h and examined using a Tescan VEGA 3 scanning electron microscope (Brno, Czech Republic). The particle size and zeta potential in colloidal solutions were measured using a Photocor EPM/Photocor Compact Z (Moscow, Russia). The surface area, pore volume, and pore size distribution were investigated using Quantachrome Nova 1200e (Boynton Beach, FL, USA) by nitrogen adsorption at 77 K, and analyzed using the BET and BJH equations. Prior to analysis, all samples were degassed at 110 °C for 4 h.
7
Scanning Electron Microscopy of FLO-1 Cells
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Scanning electron microscopy was carried out as previously described35 (link),41 (link),45 (link),46 (link). FLO-1 cells cultured at the A–L and L–L interfaces on porous membranes (BD Falcon) in DMEM, A-DMEM and UroM for 1, 2 or 4 wks were fixed in 2% formaldehyde (w/v) and 2% glutaraldehyde (v/v) in 0.1 M cacodylate buffer, pH 7.4 for 2 h 45 min at 4 °C. The fixation was followed by rinsing in 0.2 M cacodylate buffer. The samples were then post-fixed in 1% (w/v) osmium tetroxide for 2 h at room temperature, dehydrated in a graded series of ethanol, dried at a critical point, spattered with gold and examined at 30 kV with a Tescan Vega3 scanning electron microscope (Brno, Czech Republic).
8
Tissue Fixation and Preparation for SEM
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The tissue from the ex vivo and in vivo experiments was fixed in a mixture of 2% paraformaldehyde (Merck KgaA, Germany) and 2% glutaraldehyde (Serva, Germany) in 0.1 M cacodylate buffer (pH 7.4; Serva, Germany) for 3 to 4 h at 4 °C. The tissue samples were then rinsed in 0.1 M cacodylate buffer and post-fixed in 1% osmium tetroxide (Serva, Germany) in the same buffer for 1 h at 4 °C. Specimens were critical-point dried, attached to aluminum holders with silver paste (Silver DAG1415, Plano GmbH, Germany), sputter-coated with gold, and examined in a Tescan Vega3 scanning electron microscope at 30 kV (Brno, Czech Republic).
9
Fracture Morphology Analysis of Composite Materials
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In order to analyze the fracture morphology and microstructure of the specimens before and after mechanical testing, TESCAN VEGA 3 Scanning Electron Microscope (TESCAN, Czechoslovakia) was utilized. To reveal the cross-section through the thickness, samples taken from the laminates were mounted in acrylic resin. A total of five samples were selected for examination, including one jute-cotton yarn, one pineapple-cotton yarn, a pineapple-cotton fibre reinforced epoxy polymer composite (PFRP), a jute-cotton fibre reinforced epoxy polymer composite (JFRP), and a cotton blended pineapple-jute fibre reinforced hybrid composite. The yarns used in the experiment were derived from the fabrics employed, while the composite samples were obtained by CO2 laser cutting from the fractured portions of the tensile testing specimens, with a dimension of 10 mm. These samples were then mounted onto a sample holder. Prior to SEM imaging, the specimens were sputter-coated with gold dust for 1 min. This coating helps to improve the conductivity of the sample and minimize charging effects during imaging, enabling better quality SEM images to be obtained.
10
Multimodal Lung Tissue Analysis
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For LM, pieces of the lung were processed by standard tissue processing techniques201 . Tissues were embedded in paraffin wax, the blocks cut at 7 μm thickness and the sections stained with hematoxylin and eosin. For each block, the first technically adequate section was used for LM study and morphometric analysis at that level. For TEM, the pieces of lung tissue were processed by conventional laboratory techniques201 and ultra-thin sections cut and stained with uranyl acetate and lead citrate for viewing on a Philips CM 10 TEM microscope at an accelerating voltage of 80 kV. For SEM, samples of the lung tissue were dehydrated with ethanol and exposed to two changes of hexamethyldisilazine (HMDS) (Sigma-Aldrich, St. Louis, MO, U.S.A). The samples were sputtered with gold-palladium complex and viewed on a TESCAN® VEGA3 scanning electron microscope (Brno, Czech Republic) at an accelerating voltage of 8 kV.
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