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G1281

Manufactured by Solarbio
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Sourced in China

G1281 is a laboratory equipment designed for general use in research and analysis applications. It serves as a versatile tool for various laboratory tasks. The core function of G1281 is to provide a reliable and consistent performance in the laboratory environment.

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Lab products found in correlation

G1120, by Solarbio (2 mentions) G1220, by Solarbio (1 mentions) G1346, by Solarbio (1 mentions) G1140, by Solarbio (1 mentions) Paraformaldehyde fix solution, by Wuhan Servicebio Technology (1 mentions) G1100, by Solarbio (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Rm2235, by Leica (1 mentions) G1472, by Solarbio (1 mentions) Image pro plus, by Media Cybernetics (1 mentions)

13 protocols using g1281

1

Histological Analysis of Intestinal and Liver Tissues

Cited 2 times
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The proximal and distal small intestines and liver were excised and fixed in 4% paraformaldehyde for 48 h and then embedded in paraffin. The sections were sliced at a thickness of 3–5 μm and stained with hematoxylin and eosin (H&E) in accordance with the manufacturer's instructions (Baso).
For Oil Red O staining, the proximal small intestines samples were fixed in 4% paraformaldehyde for 48 h, then immersed in 15% and 30% sucrose in PBS for 12 h each, and subsequently embedded in OCT (SAKURA). The sections were cut at a thickness of 8–10 μm and stained according to the manufacturer's instructions (Baso, BA-4081).
Immunohistochemistry and immunofluorescence staining were performed as described previously by incubating the sections with primary antibodies at 4 °C overnight, followed by incubation with secondary antibodies at room temperature for 1 h [32 (link),33 (link)]. The antibodies were listed in Table S4.
Periodic Acid Schiff (PAS) staining was performed in accordance with the manufacturer's instructions (Solarbio, G1281).
Guo C.G., Sun R., Wang X., Yuan Y., Xu Y., Li S., Sun X., Wang J., Hu X., Guo T., Chen X.W., Xiao R.P, & Zhang X. (2023). Intestinal SURF4 is essential for apolipoprotein transport and lipoprotein secretion. Molecular Metabolism, 79, 101847.
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2

Myocardial Glycogen Content Analysis

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To examine the hearts’ metabolic state, glycogen and other polysaccharide contents of the myocardia were assessed by performing PAS staining described by the manufacturer (Solarbio; G1281). PAS-positive areas were observed at X40 magnification and quantified with ImageJ.
Adzika G.K., Mprah R., Rizvi R., Adekunle A.O., Ndzie Noah M.L., Wowui P.I., Adzraku S.Y., Adu-Amankwaah J., Wang F., Lin Y., Fu L., Liu X., Xiang J, & Sun H. (2023). Occlusion preconditioned mice are resilient to hypobaric hypoxia-induced myocarditis and arrhythmias due to enhanced immunomodulation, metabolic homeostasis, and antioxidants defense. Frontiers in Immunology, 14, 1124649.
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3

Tissue Histological Characterization

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The sections were deparaffinized and stained with hematoxylin and eosin (HE staining; BA4025, BaSO Diagnostics Inc., Zhuhai, China), periodic acid–Schiff (PAS) (G1281, Solarbio, Beijing, China) staining was used to detect lipoprotein deposition, and observed under a light microscope. Collagen accumulation was assessed by morphometric analysis of Van Gieson staining (VG staining; BA4084, BaSO Diagnostics Inc., Zhuhai, China) according to the manufacturer’s instructions.
Li Y., Jin F., Li T., Yang X., Cai W., Li S., Gao X., Mao N., Liu H., Xu H, & Yang F. (2022). Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation. Veterinary Sciences, 9(6), 251.
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4

Glycogen Storage and Binucleation Visualization

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Cells in culture dishes were fixed in cold methanol, and glycogen storage was visualized by PAS staining (Solarbio, G1281, Beijing, China). Binucleated cells were observed by H & E staining using a kit (Beyotime, C0105S, Jiangsu, China) following the manufacturer’s instructions, and images were taken with a microscope.
Xie X., Zhou X., Liu T., Zhong Z., Zhou Q., Iqbal W., Xie Q., Wei C., Zhang X., Chang T.M, & Sun P. (2022). Direct Differentiation of Human Embryonic Stem Cells to 3D Functional Hepatocyte-like Cells in Alginate Microencapsulation Sphere. Cells, 11(19), 3134.
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5

Histological Analysis of Kidney Injury

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For histological analysis, the paraffin-embedded kidney was sliced into 2-µm sections, which were subjected to hematoxylin and eosin (H&E) staining (G1120, Solarbio, China), or periodic acid–Schiff staining (G1281, Solarbio, China) according to the manufacturer’s instructions. Tubule injury scores were determined by grading tubular dilatation, tubular casting formation, cell necrosis, and brush edge loss. At least 10 fields viewed using a microscope (×400) were randomly selected and scored as follows: 0, no damage; 1, less than 25%; 2, 25%–50%; 3, 50%–75%; 4, >75% (Zhang et al., 2020 (link)).
Zhang M., Dong R., Da J., Yuan J., Zha Y, & Long Y. (2022). Hyperhomocysteinemia exacerbates acute kidney injury via increased mitochondrial damage. Frontiers in Physiology, 13, 967104.
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6

Histopathological Analysis of Lung Tissue

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After the mice were killed, part of the lung tissue was fixed in 4% paraformaldehyde, and the other part was stored at −80 °C. The fixed lung tissues were dehydrated and embedded in paraffin. Paraffin sections (3 μm) were dewaxed and rehydrated for hematoxylin and eosin (H&E) staining and periodic acid–Schiff reagent (PAS) staining. Procedures of PAS staining were carried out according to the kit instruction (G1281, Solarbio). We also analyzed the expression of NK1R in lung tissues by immunohistochemical staining. Primary antibody anti-neurokinin 1 receptor antibody was diluted at a ratio of 1:150. After staining, the infiltration of inflammatory cells and mucus secretion were observed under the microscope (100×). NK1R-positive expression was also observed and analyzed by Image J software.
Zhang J., Zhang C., Miao L., Meng Z., Gu N, & Song G. (2023). Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor. Pharmaceutical Biology, 61(1), 449-458.
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7

Kidney Histological Assessment via PAS and Sirius Red

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Before proceeding with the staining protocol, the kidney was fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned at a thickness of 4 μm with a slicer. After deparaffination and rehydration, the slides were stained with PAS (G1281, Solarbio, China) or Sirius Red (BA4079B, BASO, China). Images were captured at 200× magnification by an Olympus microscope (Tokyo, Japan). The renal injury index of pathological lesions in the PAS assay was scored according to previous reports (Weidemann et al. 2008 (link)). The relative Sirius Red-stained fibrotic area in the renal cortex was analyzed using a double-blind method (Turnberg et al. 2006 (link)).
Song J., Wang H., Sheng J., Zhang W., Lei J., Gan W., Cai F, & Yang Y. (2023). Vitexin attenuates chronic kidney disease by inhibiting renal tubular epithelial cell ferroptosis via NRF2 activation. Molecular Medicine, 29, 147.
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8

Histological Evaluation of Renal Fibrosis

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For histological observation of renal morphology, the renal tissues were fixed in 4% buffered paraformaldehyde, embedded in paraffin, and sliced at 3 mm. In accordance with the protocol, hematoxylin and eosin staining (HE; Solarbio, G1220), Masson trichrome staining (Solarbio, G1346), and periodic acid-schiff staining (PAS; Solarbio, G1281) were performed, and the stained sections were analyzed using a computational color image analysis system (Leica Microscope, Germany DM2500). Utilizing ImageJ software, the collagen tissue area was calculated to assess renal fibrosis quantitatively.
Peng R., Zuo S., Li X., Huang Y., Chen S., Zou X., Long H., Chen M., Yang Y., Yuan H., Zhao Q., Guo B, & Liu L. (2024). Investigating HMGB1 as a potential serum biomarker for early diabetic nephropathy monitoring by quantitative proteomics. iScience, 27(2), 108834.
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9

Quantifying Lung Goblet Cell Metaplasia

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The paraffin‐embedded lung tissue was sectioned (4 mm thick) and subsequently subjected to dehydration in alcohol. Periodic acid‐Schiff (PAS) staining was performed following the manufacturer's instructions (purchased from Solarbio, catalog number G1281). The assessment of mucus secretion in lung tissue was conducted based on the scoring system described in previously reported literature.
32 (link) The PAS staining scoring criteria were as follows: Each airway was scored based on the percentage of surrounding goblet cells. A score of 0 represented ≤5% goblet cells, a score of 1 indicated 5‐25% goblet cells, a score of 2 denoted 25‐50% goblet cells, a score of 3 indicated 50‐75% goblet cells, and a score of 4 represented ≥75% goblet cells.
32 (link)
Meng H., Zhang D., Que Y., Hu P., Wang R., Liao Y, & Xu G. (2024). Intermittent hypoxic pretreatment exacerbates house dust mite‐induced asthma airway inflammation. Immunity, Inflammation and Disease, 12(4), e1253.
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10

Histological Analysis of Mouse Vaginal Tissue

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Mouse vaginal tissues were fixed in Paraformaldehyde Fix Solution (G1101-500ML, Servicebio) for 24 h and embedded in paraffin, and sectioned at 5 μm. Sections were dried at 60 °C for 30 min, deparaffinized and dehydrated in ddH2O; 75% ethanol, 85% ethanol, 90% ethanol, 100% ethanol, and water. Sections of the vagina were stained with hematoxylin (G1140, Solarbio) and eosin (G1100, Solarbio). Cornification of vaginal epithelial cells was identified using PAS staining (G1281, Solarbio). The TUNEL assay was performed on paraffin-embedded sections of the vagina using an In Situ Cell Death Detection Kit (11684817910, Roche) according to the manufacturer’s instructions.
Wan S., Sun Y., Fu J., Song H., Xiao Z., Yang Q., Wang S., Yu G., Feng P., Lv W., Luo L., Guan Z., Liu F., Zhou Q., Yin Z, & Yang M. (2022). mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation. Cell Death & Disease, 13(10), 862.
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