Behavioral training and testing for the 5CSRTT was conducted in four identical mouse Bussey-Saksida touchscreen operant chambers (Lafayette Instruments Co., Lafayette, IN). Each apparatus consisted of a sound attenuating chamber with a fan for ventilation/ background noise reduction enclosing a trapezoidal operant area with black plastic walls, a perforated stainless-steel floor, and a clear plexiglass roof. A speaker and LED houselight were attached to the ceiling of the sound attenuation chamber, directly above the operant arena (the houselight was off unless otherwise specified). A touchscreen (30.7 cm, 800 × 600 resolution) located at the front of the arena was covered by a black plastic mask with 5 square openings (4 × 4 cm each, spaced 1 cm apart, 1.5 cm above floor; ‘5 choice’ mask; Lafayette Instruments Co., Lafayette, IN) to define response areas and reduce accidental background touches. A feeder with an LED light was located at the back end of the chamber, with undiluted evaporated milk (Nestle Carnation) reward delivered by a liquid pump. Infrared beams were positioned at the front and back of the chamber, as well as in the feeder. Stock behavioral programs (5-Choice Serial Reaction Time Task for Mouse Touch Screen Systems and ABET II) were executed by the ABET II software (Lafayette Instruments Co., Lafayette, IN) and Whisker Server (Cardinal & Aitken, 2010 (link)).
Abet 2 software
Manufactured by Lafayette Instrument
ABET II software is a comprehensive tool designed for the assessment and evaluation of academic programs. It provides a standardized framework for collecting, analyzing, and reporting data related to program outcomes and accreditation requirements.
Automatically generated - may contain errors
Lab products found in correlation
Triangular touchscreen operant chambers, by Lafayette Instrument (4 mentions)
Model 80015ns, by Lafayette Instrument (2 mentions)
Bussey saksida touchscreen operant chambers, by Lafayette Instrument (1 mentions)
Whisker server, by Lafayette Instrument (1 mentions)
Abet 2, by Lafayette Instrument (1 mentions)
Modular operant conditioning chambers, by Med Associates (1 mentions)
Mouse touch screen chambers, by Lafayette Instrument (1 mentions)
Env 007, by Med Associates (1 mentions)
Env 018v, by Med Associates (1 mentions)
Map data acquisition system, by Plexon (1 mentions)
13 protocols using abet 2 software
1
5-Choice Serial Reaction Time Task for Mice
2
Visuo-Cognitive Task in Rat Operant Chambers
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The visuo-cognitive tasks were carried out in two rat operant chambers with touchscreen capabilities (Campden Instruments Ltd.). Each chamber was individually controlled and located in noise-attenuation boxes. The routines controlling the experimental events were programmed using ABET II Software (Lafayette Instrument Company, Inc.) and managed using Whisker [18 (link)]. Visual stimuli were displayed in 4 different 6.6 × 6.6 cm areas. Two, vertically aligned locations were used to present sample stimuli, and the two remaining horizontally aligned locations were used to present response stimuli. A pellet-dispenser delivered sucrose pellets (5TUL; TestDiet) into a food cup located on the wall opposite to the touchscreen.
Following a reduction to 85% of their free-feeding weights, rats received hand-shaping to operate the touchscreen. After touchscreen performance was satisfactory, each subject received a pretraining session containing 100 trials. On each trial, an image appeared randomly in any of the four possible locations (top, bottom, left, or right). After the subject touched the image, a set of simultaneous events associated with the delivery of the reward ensued: the image disappeared from the screen, a pure tone (1 kHz) was played inside the chamber for 1 s, and 2 food pellets were delivered into the food cup. The interval between trials varied randomly between 6 and 10 s.
Following a reduction to 85% of their free-feeding weights, rats received hand-shaping to operate the touchscreen. After touchscreen performance was satisfactory, each subject received a pretraining session containing 100 trials. On each trial, an image appeared randomly in any of the four possible locations (top, bottom, left, or right). After the subject touched the image, a set of simultaneous events associated with the delivery of the reward ensued: the image disappeared from the screen, a pure tone (1 kHz) was played inside the chamber for 1 s, and 2 food pellets were delivered into the food cup. The interval between trials varied randomly between 6 and 10 s.
3
Operant Conditioning Chamber Design for Behavioral Enrichment
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Sixteen modular operant conditioning chambers (24.1 cm in width × 20.5 cm in depth × 29.2 cm in height; MED Associates) were used for behavioral enrichment. Each chamber was in a sound-attenuating cubicle equipped with a ventilation fan to provide ambient background noise. Each chamber was equipped with a food magazine, a retractable lever (counterbalanced on the left or right side of the magazine), and a red house light on the wall opposite of the magazine. The magazine contained an infrared sensor to detect magazine entries, and the levers were calibrated to detect lever deflections in response to 10 g of applied weight. Whenever a lever was extended into the chamber, an LED mounted inside the lever mechanism illuminated the slot through which the lever protruded (ABET II Software; Lafayette Instrument).
4
Reward-Driven Task for Mice Screening
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Prior to surgeries, both photometry and optogenetic cohorts mice were screened in this simple reward delivery task. Mice were habituated to the experimental chambers (Mouse Touch Screen Chambers installed in sound-proof chambers, Lafayette Instruments) for 20 min per day for two days. The task schedule was designed and controlled by ABET II software (Lafayette Instrument). On three consecutive days, mice could receive up to 15 milkshake deliveries (Energy milk, strawberry flavor, Emmi Schweiz AG; 20 μL/delivery which was consistently entirely eaten by all mice; mice had been previously habituated in the home cage) during a maximum of 30 min. The session ended when whichever of the 15 deliveries or 30 min came first. The inter-trial interval varied pseudo-randomly, 30 ± 10 sec, counted from the moment a mouse exited the food magazine. Only those able to perform the task in 10 min or less were implanted. This was an indication that mice made the association between the cue and food delivery. The house light (white light) was on during the sessions. The tray light was turned on at each milkshake delivery and turned off when the mouse visited the food magazine. Milkshake delivery was associated with a sound cue. Consumption onset was defined as the first food magazine IR beam break following each delivery.
5
Triangular Touchscreen Operant Chambers
6
Touchscreen Operant Conditioning Protocol
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16 identical triangular touchscreen operant chambers (Lafayette Instrument Co., Lafayette, IN) were used for training and testing. The touchscreen was housed in the front while the food delivery magazine in the back. Information on individual touches on touchscreens throughout sessions were recorded via ABET-II software. Touchscreens were limited by masks with holes which allowed responding in 5 square holes. Liquid reinforcer (50% diluted Ensure) was pumped via a peristaltic pump (1000 ms or 250 ms duration, corresponding to volumes of approximately 25 μl and 6.25 μl). ABET-II software (Lafayette Instrument Co., Lafayette, IN) was used to program operant schedules and analyze all data from training and testing.
7
Operant Conditioning with Colored Stimuli
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Two identical operant chambers (Med Associates, ENV-007), measuring 28.5 cm (height) × 30 cm (length) × 24 cm (width), were used. On the response panel, three keys (2.5 cm in diameter) were horizontally arranged, 8 cm center-to-center apart from one another. The bottom of each key was 20.5 cm above the steel grid floor, and every key was equipped with a 12-stimulus projector (Med Associates, ENV-130M). The food, mixed grain, was delivered via a LVE (Lehigh Valley Electronics) food hopper, and was accessible through a 6.5-cm wide × 5-cm high opening, centered horizontally on the response panel, 4 cm above the floor. When the hopper was raised, a 28-V, 0.04-A light illuminated its opening. On the wall opposite to the response panel (26 cm above the floor) a 28-V, 0.1-A houselight provided general illumination. The operant chamber was inside a PVC sound attenuating cubicle (Med Associates, ENV-018V), equipped with an exhaust fan that circulated air through the chamber and masked outside noises.
In this experiment, red or green hues were presented on the side keys and a white hue or a white cross (+) on a black background were presented on the center key.
Personal computers running the ABET II software (Lafayette Instrument Company) controlled the experimental events and recorded the data.
In this experiment, red or green hues were presented on the side keys and a white hue or a white cross (+) on a black background were presented on the center key.
Personal computers running the ABET II software (Lafayette Instrument Company) controlled the experimental events and recorded the data.
8
Multimodal Recording of Neural Activity
9
Triangular Operant Chambers for Behavioral Training
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Sixteen identical triangular touchscreen operant chambers (Lafayette Instrument Co., Lafayette, IN) were used for training and testing. Two walls were black acrylic plastic. The third wall housed the touchscreen and was positioned directly opposite the magazine. The magazine provided liquid reinforcer (Ensure) delivered by a peristaltic pump, typically 7 µl (280 ms pump duration). ABET-II software (Lafayette Instrument Co., Lafayette, IN) was used to program operant schedules and to analyze all data from training and testing.
10
Operant Conditioning of Pigeons in Soundproof Chambers
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Three Med Associates operant boxes for pigeons were used. The boxes were 28.5-cm high, 24-cm long, and 30-cm wide. Each box was enclosed in a sound-attenuating chamber, equipped with a fan that circulated air and masked extraneous noises. The response panel had three circular keys, each 2.5 cm in diameter, and placed 6 cm apart (center-to-center), with the lowest edge 21 cm above the floor grid. The response panel also included a 6-cm wide × 5-cm high opening, centered 4 cm above the floor grid. The pigeon had access to food when the opening was illuminated by a 1.1-W light and the food hopper was raised. In the panel opposite to the response panel, a houselight (2.8 W) centrally located 23 cm above the floor illuminated the whole box. A personal computer with ABET II software (Lafayette Instruments) controlled the events and recorded data. Communications with the experimental chambers used the Whisker interface (Cardinal and Aitken 2010 (link)).
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