A Shimadzu spectrofluorophotometer RF-6000 was used to record the fluorescence spectra of a silk-fibroin film. Fluorescence excitation-emission matrices were measured using an excitation wavelength of 256 nm. The corresponding emission in the 250–600 nm range with 5 nm increment was recorded. These wavelength ranges supported for intrinsic fluorescence measurements of tyrosine, tryptophan and other endogenous fluorophores in the UV-Vis range. The photomultiplier tube detector gain was fixed at 700 V.
Rf 6000 spectrofluorophotometer
Manufactured by Shimadzu
Sourced in Japan, United States
The Shimadzu RF-6000 spectrofluorophotometer is a compact and versatile instrument designed for fluorescence analysis. It features a monochromator-based optical system and a high-performance photomultiplier tube detector. The RF-6000 is capable of measuring the fluorescence excitation and emission spectra of various samples.
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Lab products found in correlation
Uv 1800 spectrophotometer, by Shimadzu (3 mentions)
Cary 60 uv vis spectrophotometer, by Agilent Technologies (3 mentions)
Fiveeasy ph meter, by Mettler Toledo (2 mentions)
Lambda 750 uv vis nir spectrometer, by PerkinElmer (1 mentions)
Ce 440, by PerkinElmer (1 mentions)
Su 8010 sem instrument, by Hitachi (1 mentions)
Maldi tof ms performance, by Shimadzu (1 mentions)
Spectrum 100 ftir spectrometer, by PerkinElmer (1 mentions)
400 mhz spectrometer, by Bruker (1 mentions)
Chi 660d, by CH Instruments (1 mentions)
Zetasizer nano s equipment, by Malvern Panalytical (1 mentions)
1200 series instrument, by Agilent Technologies (1 mentions)
Black flat bottom 96 well microplates, by Corning (1 mentions)
Thermo k alpha, by Thermo Fisher Scientific (1 mentions)
U 2900 uv vis spectrophotometer, by Hitachi (1 mentions)
Zetasizer ultra system, by Malvern Panalytical (1 mentions)
Labsolutions rf software, by Shimadzu (1 mentions)
Nexsa spectrometer, by Thermo Fisher Scientific (1 mentions)
Cary 630 ftir, by Agilent Technologies (1 mentions)
Tma dph, by Merck Group (1 mentions)
46 protocols using rf 6000 spectrofluorophotometer
1
Fluorescence Characterization of Silk Fibroin
2
Characterization of novel HQTPE compound
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All 1H and 13C NMR spectra were recorded in DMSO-d6 solutions at 298 K using a Bruker (400 MHz) spectrometer with tetramethylsilane as an internal standard. Mass spectra were recorded on matrix-assisted laser desorption ionization time-of-flight MS (MALDI-TOF MS), Performance (Shimadzu, Japan). Perkin Elmer CE-440 was used for micro elemental analyses. Infrared spectra were recorded by Perkin Elmer (Spectrum 100) FT-IR Spectrometer. UV-vis spectra were recorded using Perkin Elmer (Lambda 750) UV/Vis/NIR Spectrometer in ethyl acetate solvent. Fluorescence emission, quantum yield, and lifetime measurements were performed by SHIMADZU Spectro fluorophotometer RF-6000. Aggregations of HQTPE were examined by scanning electron microscopy (SEM) by a Hitachi SU-8010 SEM instrument fitted with a field-emission source and operating at 10 kV. Samples were prepared for SEM by mounting a portion of the cover slide on a silicon stub. Electrochemical measurements were performed on a CH-Instruments Model CHI660D using a one-compartment cell under air atmosphere. The three-electrode system consisted of a platinum counter electrode, an Ag/AgCl reference electrode, and a platinum working electrode.
3
Binding Study of Capsaicin-HTF Complex
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We employed fluorescence binding study to check the actual binding of capsaicin with htf in line with earlier studies on Shimadzu Spectrofluorophotometer (RF-6000). The concentration of htf was kept constant (4 µM) and capsaicin was varied in the range of 0–6 µM. The data obtained was put into Modified Stern–Volmer (MSV) equation as per earlier literature to obtain the binding constant (K) of Htf-capsaicin complex (Shamsi et al., 2020 (link)). We carried out the experiment in triplicates and mean value was taken into account. All the spectra reported here are the subtracted spectra after considering the fluorescence of capsaicin by taking a blank spectra of the ligand.
4
Spectrofluorimetric Analysis Equipment
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Shimadzu spectrofluorophotometer RF-6000 (Kyoto,Japan) equipped with 150 W Xenon lamp and 1.0 cm quartz cell was used for spectrofluorimetric measurements. The spectral bandwidth for both monochromators was set at 5 nm with auto adjustment of the sensitivity of detector. The spectrofluorophotometer is connected to Lenovo computer loaded with LabSolutions RF software, Thermo Scientific™ Megafuge (UK). Sartorius Secura 324-1S analytical balance (Germany), vortex V2H (Boeco, Germany) and ultrasonic water bath (R. Espinar S.L, Spain) were used.
5
Relative Fluorescence Intensity Measurements
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Shimadzu spectrofluorophotometer RF-6000 was used during all relative fluorescence intensity measurements (serial no. A402458, Shimadzu Corporation, Kyoto, Japan).
Jenway 3520 pH/temp meter, combined with glass pH electrode was used for pH adjustment (Jenway, Staffordshire, United Kingdom).
Jenway 3520 pH/temp meter, combined with glass pH electrode was used for pH adjustment (Jenway, Staffordshire, United Kingdom).
6
Tyrosinase Fluorescence Quenching by Kojic Acid
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Steady state fluorescence spectra were carried out on a Shimadzu spectrofluorophotometer RF-6000 (Kyoto, Japan). All measurements were performed in a standard quartz cell of 1.0 cm path length, embedded in a thermostatic cell holder. Fluorescence emission spectra were recorded upon excitation wavelength at 280 nm. The slit widths for excitation and emission were set to 3 nm and 5 nm, respectively. A 3 ml solution in cuvette containing of 0.65μM tyrosinase, within the linear concentration region for fluorescence intensity (data not shown), was added successively with 1μl of 30 mM kojic acid aliquot using a trace syringe for 10 times. The addition of small volume of buffer solution during titration had little effect on the extent of fluorescence quenching [5, 6] (link) . Fluorescence quenching was analyzed by Stern-Volmer equation (1) [7, (link)8] (link):
(1) where F 0 and F represent the fluorescence intensities Vol-4 Issue-2
(1) where F 0 and F represent the fluorescence intensities Vol-4 Issue-2
7
Intracellular ROS Quantification by Spectrofluorescence
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Intracellular ROS were determined by oxidation of to rhodamine by spectrofluorescence. This probe measures levels of ROS intermediates such as peroxynitrite, H 2 O 2 and the hydroxyl radical OH. (Royall and Ischiropoulos 1993) . Briefly, MG-63 cells were incubated at 37 °C with different concentrations of the complexes. After 24 h, cells were incubated with 10 mM DHR-123. After 1 h, cells were scraped into 1 mL 0.1% Triton-X100. The cell extracts were then analyzed for the oxidized product rhodamine by measuring fluorescence (excitation wavelength, 495 nm; emission wavelength, 532 nm), using a Shimadzu Spectrofluorophotometer RF-6000 equipped with a computer working with LabSolutions RF software. Results were corrected for protein content with the Pierce TM BCA Protein Assay Kit.
8
Optical and Colloidal Characterization of M-SPIONs
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The optical characterization of M-SPIONs dispersed in aqueous medium was performed at 50 µg Fe/mL using an RF-6000 spectrofluorophotometer (Shimadzu, Kyoto, Japan) to acquire the spectrum with excitation in the wavelength range from 520 to 800 nm and emission from 550 to 780 nm.
The polydispersion of hydrodynamic diameter (HD), temporal stability of HD and zeta potential of M-SPIONs (50 µg Fe/mL) were measured using dynamic light scattering (DLS) with Zetasizer Nano S equipment (Malvern, Reino Unido). The HD distribution curve was obtained using an angle of 173 degrees with 15 measurements in 5 s, maintaining a constant temperature at 37 °C. M-SPION HD stability analysis was performed in cell culture medium (DMEM +10% FBS) for 20 h. The zeta potential measurements (surface charge) were performed in the pH range from 7 to 9.
The polydispersion of hydrodynamic diameter (HD), temporal stability of HD and zeta potential of M-SPIONs (50 µg Fe/mL) were measured using dynamic light scattering (DLS) with Zetasizer Nano S equipment (Malvern, Reino Unido). The HD distribution curve was obtained using an angle of 173 degrees with 15 measurements in 5 s, maintaining a constant temperature at 37 °C. M-SPION HD stability analysis was performed in cell culture medium (DMEM +10% FBS) for 20 h. The zeta potential measurements (surface charge) were performed in the pH range from 7 to 9.
9
Spectroscopic and Chromatographic Analyses of Enzymatic Reactions
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3D scans were performed on a Shimadzu RF 6000 spectrofluorophotometer. Enzyme activity by UV, fluorescence measurements and OD600 determinations were performed using a Tecan Spark 10 M microplate reader. Enzyme activity characterization through conversions was performed by HPLC measurements, using an Agilent Technologies 1200 Series instrument equipped with autosampler. The UV irradiation of assay samples was performed in Corning 96-well Clear Flat Bottom UV-Transparent microplates using a handheld Analytik Jena UV lamp of 302 nm. The fluorescence measurement was carried out in Corning 96-well Black Flat Bottom microplates. Styrene was purchased from Sigma Aldrich and solvents (n-hexane, n-octane, dimethyl sulfoxide, acetonitrile) of HPLC grade were purchased from VWR. The synthesis of fluorogenic probe 4 was performed according to reported procedure47 (link).
10
Fluorescence-based Determination of Cu(II) Complexation by Humic Substances
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The Cu(II) complexing capacities of HAn and HA enz as well as the stability constants of the Cu(II)-HA complexes were determined by the quenching of fluorescence according to the method proposed by Ryan and Weber (1982) (link) and the methodology detailed in Plaza et al. (2005) (link) and Fuentes et al. (2013) (link). Briefly, stock solutions of 30 mg HA or HA enz L-1 were prepared in 0.1M KNO3 and the pH was adjusted to 8. Aliquots of the stock solutions were diluted with equal volumes of solutions of different concentrations of Cu(NO3)2 in 0.1M KNO3 at pH 8, so that the final solutions had 15 mg L-1 HA or HA enz, 0.1M KNO3 and 0, 7.5, 15, 25, 40 or 60 µM Cu(II). Solutions were left overnight at room temperature before fluorescence measurements. Fluorescence measurements were performed on a Shimadzu RF-6000 Spectrofluorophotometer.
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